Detailed information of TA system TA113364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 325902..326491
• Replicon: chromosome
• Accession: NZ_CP033192
• Organism: Xanthomonas oryzae pv. oryzae strain NX0260

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: EBA21_RS01520
• Protein ID: WP_012443754.1
• Coordinates: 325902..326183 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBA21_RS01525
• Protein ID: WP_011260746.1
• Coordinates: 326201..326491 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA21_RS01515	323555..325807	+	2253	WP_012443753.1	exodeoxyribonuclease V subunit alpha	-
EBA21_RS01520	325902..326183	+	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBA21_RS01525	326201..326491	+	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
EBA21_RS01535	327420..328814	+	1395	WP_011260745.1	nucleoside hydrolase	-
EBA21_RS01545	329264..329596	-	333	WP_011260743.1	hypothetical protein	-
EBA21_RS01555	329932..330177	+	246	WP_069960049.1	hypothetical protein	-
EBA21_RS01560	330293..330727	+	435	WP_011260742.1	PAAR domain-containing protein	-
EBA21_RS01565	330730..331206	+	477	WP_011260741.1	DUF4123 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	307240..347806	40566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T113364 WP_012443754.1 NZ_CP033192:325902-326183 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T113364 NZ_CP033192:325902-326183 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT113364 WP_011260746.1 NZ_CP033192:326201-326491 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT113364 NZ_CP033192:326201-326491 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5