Detailed information of TA system TA113363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4717943..4718532
• Replicon: chromosome
• Accession: NZ_CP033191
• Organism: Xanthomonas oryzae pv. oryzae strain PXO364

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: EBA22_RS23465
• Protein ID: WP_012443754.1
• Coordinates: 4718251..4718532 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBA22_RS23460
• Protein ID: WP_011260746.1
• Coordinates: 4717943..4718233 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA22_RS23420	4713231..4713704	-	474	Protein_4208	DUF4123 domain-containing protein	-
EBA22_RS23425	4713707..4714141	-	435	WP_011409747.1	PAAR domain-containing protein	-
EBA22_RS23430	4714257..4714502	-	246	WP_069960049.1	hypothetical protein	-
EBA22_RS23440	4714838..4715170	+	333	WP_011260743.1	hypothetical protein	-
EBA22_RS23450	4715620..4717014	-	1395	WP_027703652.1	nucleoside hydrolase	-
EBA22_RS23460	4717943..4718233	-	291	WP_011260746.1	HigA family addiction module antidote protein	Antitoxin
EBA22_RS23465	4718251..4718532	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EBA22_RS23470	4718627..4720879	-	2253	WP_069960050.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T113363 WP_012443754.1 NZ_CP033191:c4718532-4718251 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T113363 NZ_CP033191:c4718532-4718251 [Xanthomonas oryzae pv. oryzae]
GTGATCAGGAGCTTTATCGACAAGGACGCCGAAAAGATCTGGCTGGGTGAGCGTTCCCGCCGGTTGCCTGCCGACATCCA
ATTGGTCGCGCGCCGCAAGTTGCGCATGCTCAATGCCGCCGCACATCTCGACGATCTGCGGATCCCGCCCGCCAACCGGC
TGGAAGCGTTGAAGGGCCAGCGGCGCGGCCAGTACAGCATCCGGATCAACGATCAATGGCGCATCTGCTTCCGATGGATG
GAAGGCGATGTGGTCCAGGTCGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT113363 WP_011260746.1 NZ_CP033191:c4718233-4717943 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT113363 NZ_CP033191:c4718233-4717943 [Xanthomonas oryzae pv. oryzae]
ATGACGGTCCTACCCAATATCCATCCTGGCGACATTCTGCTTGAGGAGTTTCTCGAGCCTCTGGGCATCAGCCAGAATGC
ACTGGCACGCGCCACCGGCGTCCCGCCGCGCCGTATCAACGAAATCGTGCTGGGCAAGCGCGGCATCACTGCCGACACTG
CCGTGCGTCTGGCCGCCGCGTTGGGCACCTCCGAGCGCTTCTGGCTGGGCCTGCAGGCCGACTACGAGCTGGAACAGGCG
CATCGCGCGCTGGGCGATTTGCCGGCACGGATCAAGCGGCTGGCGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5