Detailed information of TA system TA113308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4785264..4785994
• Replicon: chromosome
• Accession: NZ_CP033162
• Organism: Serratia sp. P2ACOL2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A515D591
• Locus tag: EBA31_RS23005
• Protein ID: WP_041415720.1
• Coordinates: 4785264..4785575 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EBA31_RS23010
• Protein ID: WP_020831880.1
• Coordinates: 4785572..4785994 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EBA31_RS22975	4780549..4781274	+	726	WP_020831867.1	LPS export ABC transporter ATP-binding protein	-
EBA31_RS22980	4781325..4782758	+	1434	WP_020831868.1	RNA polymerase factor sigma-54	-
EBA31_RS22985	4782782..4783069	+	288	WP_004948547.1	ribosome hibernation promoting factor	-
EBA31_RS22990	4783228..4783689	+	462	WP_115060613.1	PTS IIA-like nitrogen regulatory protein PtsN	-
EBA31_RS22995	4783821..4784675	+	855	WP_012147065.1	RNase adapter RapZ	-
EBA31_RS23000	4784672..4784944	+	273	WP_020831874.1	PTS phosphocarrier protein NPr	-
EBA31_RS23005	4785264..4785575	+	312	WP_041415720.1	type II toxin-antitoxin system HigB family toxin	Toxin
EBA31_RS23010	4785572..4785994	+	423	WP_020831880.1	helix-turn-helix domain-containing protein	Antitoxin
EBA31_RS23015	4786011..4787927	-	1917	WP_116691073.1	BglG family transcription antiterminator	-
EBA31_RS23020	4787979..4789196	-	1218	WP_122080299.1	lactonase family protein	-
EBA31_RS23025	4789348..4790088	-	741	WP_122080300.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12299.20 Da
• Isoelectric Point: 10.4493

>T113308 WP_041415720.1 NZ_CP033162:4785264-4785575 [Serratia sp. P2ACOL2]
MHIISREPFNEAAIRYPNEAASLNTIYKTLRRGTFHTPEDLKSLFPSLDRMKYKAKWWVIDVGGNHLRILFFASFDTQKI
FVKQIVTHAEYDRLLQQYRRNPK

Nucleotide

• Length: 312 bp

>T113308 NZ_CP033162:4785264-4785575 [Serratia sp. P2ACOL2]
ATGCATATAATTTCAAGGGAACCGTTCAACGAGGCCGCAATCCGCTACCCCAACGAAGCAGCCTCTCTCAACACAATCTA
CAAAACGCTGCGCCGGGGGACATTTCATACGCCGGAAGATTTAAAATCCCTTTTCCCCAGCCTGGACAGAATGAAGTACA
AAGCAAAATGGTGGGTCATTGATGTCGGAGGAAATCATCTCAGGATCTTATTCTTTGCCAGTTTTGATACCCAGAAAATA
TTTGTTAAACAGATAGTGACTCATGCAGAGTACGACAGATTACTGCAGCAGTACCGGAGGAACCCAAAATGA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15503.82 Da
• Isoelectric Point: 4.9381

>AT113308 WP_020831880.1 NZ_CP033162:4785572-4785994 [Serratia sp. P2ACOL2]
MITDALKATHELLRAIPLLGGSTSPQDYRDALALVEHLLETDERHPLIDVLAAKIVEYEDNSPDFAEFNQRIAQLPQGVA
ALSVLMEQYGLSQSDFENEIGKKSLVSQILSGKRSLTIPHIMALAKRFNLPPALFLPEAR

Nucleotide

• Length: 423 bp

>AT113308 NZ_CP033162:4785572-4785994 [Serratia sp. P2ACOL2]
ATGATCACCGATGCGCTTAAGGCCACCCATGAGCTGTTACGCGCCATACCCTTGTTAGGTGGCAGCACCTCGCCACAGGA
TTACCGTGATGCTCTGGCGTTAGTGGAACACCTGCTTGAAACCGACGAGCGCCACCCGCTGATTGACGTTCTGGCAGCCA
AGATCGTCGAGTATGAAGATAACAGCCCTGATTTTGCAGAGTTCAATCAGCGTATCGCACAGCTACCACAAGGTGTAGCC
GCGCTGAGCGTGCTGATGGAGCAATATGGCCTCAGTCAAAGCGACTTCGAAAACGAGATCGGCAAAAAGTCACTGGTGAG
CCAAATTCTAAGCGGAAAGCGATCGCTGACCATACCCCATATCATGGCGCTGGCAAAACGTTTCAACCTGCCGCCGGCCT
TGTTCTTGCCTGAAGCCCGTTAG

Structures

Toxin

• AlphaFold DB: A0A515D591