Detailed information of TA system TA113286

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 14083..14347
• Replicon: plasmid pESBL-EA11p1ESCUM
• Accession: NZ_CP033160
• Organism: Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: EAF06_RS24565
• Protein ID: WP_001387489.1
• Coordinates: 14083..14235 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	14287..14347 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAF06_RS24530	9429..10126	-	698	Protein_9	IS1 family transposase	-
EAF06_RS24535	10183..11517	+	1335	Protein_10	DotA/TraY family protein	-
EAF06_RS24540	11588..12250	+	663	WP_000644796.1	plasmid IncI1-type surface exclusion protein ExcA	-
EAF06_RS24545	12322..12531	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
EAF06_RS24550	12923..13099	+	177	WP_001054897.1	hypothetical protein	-
EAF06_RS24555	13164..13460	-	297	WP_011264046.1	hypothetical protein	-
EAF06_RS24560	13760..14011	+	252	WP_001291964.1	hypothetical protein	-
EAF06_RS24565	14083..14235	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	14287..14347	+	61	NuclAT_0	-	Antitoxin
-	14287..14347	+	61	NuclAT_0	-	Antitoxin
-	14287..14347	+	61	NuclAT_0	-	Antitoxin
-	14287..14347	+	61	NuclAT_0	-	Antitoxin
EAF06_RS24570	14550..14897	+	348	Protein_17	protein finQ	-
EAF06_RS24575	15083..16240	+	1158	Protein_18	IS1380-like element ISEc9 family transposase	-
EAF06_RS24580	16296..16993	+	698	Protein_19	IS1 family transposase	-
EAF06_RS24585	17007..17312	-	306	Protein_20	transcription termination factor NusG	-
EAF06_RS24590	17754..18041	-	288	WP_000074855.1	conjugal transfer protein TraA	-
EAF06_RS24595	17959..18222	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-42	-	1..62055	62055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T113286 WP_001387489.1 NZ_CP033160:c14235-14083 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T113286 NZ_CP033160:c14235-14083 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT113286 NZ_CP033160:14287-14347 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin