Detailed information of TA system TA113282

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 107716..108137
• Replicon: plasmid p1ESCUMpO83_CORR
• Accession: NZ_CP033159
• Organism: Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: EAF06_RS24250
• Protein ID: WP_001302184.1
• Coordinates: 107716..107874 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	107939..108137 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAF06_RS24215	102781..102996	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
EAF06_RS24220	103131..103778	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
EAF06_RS24225	103969..104352	-	384	WP_001063020.1	relaxosome protein TraM	-
EAF06_RS24230	104722..105294	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
EAF06_RS24235	105590..106411	-	822	WP_001234465.1	DUF945 domain-containing protein	-
EAF06_RS24240	106530..106817	-	288	WP_000107242.1	hypothetical protein	-
EAF06_RS24990	106842..107048	-	207	WP_000275854.1	hypothetical protein	-
EAF06_RS24250	107716..107874	-	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	107939..108137	-	199	NuclAT_0	-	Antitoxin
-	107939..108137	-	199	NuclAT_0	-	Antitoxin
-	107939..108137	-	199	NuclAT_0	-	Antitoxin
-	107939..108137	-	199	NuclAT_0	-	Antitoxin
EAF06_RS24255	108106..108211	-	106	Protein_125	plasmid SOS inhibition protein A	-
EAF06_RS24260	108366..108683	+	318	Protein_126	IS110 family transposase	-
EAF06_RS24265	108972..109223	-	252	Protein_127	iron-containing alcohol dehydrogenase	-
EAF06_RS24270	109308..109931	-	624	WP_000912556.1	potassium channel family protein	-
EAF06_RS24275	109947..110640	-	694	Protein_129	IS1 family transposase	-
EAF06_RS24280	110697..110801	+	105	Protein_130	thiosulfate reductase cytochrome B subunit	-
EAF06_RS24285	111047..112440	-	1394	Protein_131	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A)	-	1..146286	146286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T113282 WP_001302184.1 NZ_CP033159:c107874-107716 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T113282 NZ_CP033159:c107874-107716 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT113282 NZ_CP033159:c108137-107939 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin