Detailed information of TA system TA113279

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65009..65263
• Replicon: plasmid p1ESCUMpO83_CORR
• Accession: NZ_CP033159
• Organism: Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: EAF06_RS24005
• Protein ID: WP_032215358.1
• Coordinates: 65009..65215 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	65202..65263 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAF06_RS23975	61032..61433	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
EAF06_RS23980	61366..61623	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
EAF06_RS23985	61716..62369	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
EAF06_RS23990	63308..64165	-	858	WP_029702152.1	incFII family plasmid replication initiator RepA	-
EAF06_RS23995	64158..64232	-	75	WP_001365705.1	RepA leader peptide Tap	-
EAF06_RS24000	64468..64725	-	258	WP_000083833.1	replication regulatory protein RepA	-
EAF06_RS24005	65009..65215	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	65202..65263	+	62	NuclAT_1	-	Antitoxin
-	65202..65263	+	62	NuclAT_1	-	Antitoxin
-	65202..65263	+	62	NuclAT_1	-	Antitoxin
-	65202..65263	+	62	NuclAT_1	-	Antitoxin
EAF06_RS24985	65402..65668	-	267	WP_087757657.1	hypothetical protein	-
EAF06_RS24015	66043..66612	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
EAF06_RS24020	66764..67390	-	627	WP_029702149.1	hypothetical protein	-
EAF06_RS24025	67802..68011	-	210	WP_039023209.1	hypothetical protein	-
EAF06_RS24030	68142..68702	-	561	WP_001567328.1	fertility inhibition protein FinO	-
EAF06_RS24035	68757..69503	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12 / tet(A)	-	1..146286	146286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T113279 WP_032215358.1 NZ_CP033159:c65215-65009 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T113279 NZ_CP033159:c65215-65009 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT113279 NZ_CP033159:65202-65263 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin