Detailed information of TA system TA113273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3599208..3599902
• Replicon: chromosome
• Accession: NZ_CP033158
• Organism: Escherichia coli strain CM IVRI KOL-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: EAF06_RS17195
• Protein ID: WP_001263489.1
• Coordinates: 3599504..3599902 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: EAF06_RS17190
• Protein ID: WP_000554758.1
• Coordinates: 3599208..3599501 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAF06_RS17170	3594840..3595337	+	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
EAF06_RS17175	3595561..3597273	-	1713	Protein_3335	flagellar biosynthesis protein FlhA	-
EAF06_RS17180	3597245..3598030	+	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
EAF06_RS17185	3598101..3599156	+	1056	WP_001226164.1	DNA polymerase IV	-
EAF06_RS17190	3599208..3599501	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EAF06_RS17195	3599504..3599902	+	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EAF06_RS17200	3599912..3600364	+	453	WP_001059892.1	GNAT family N-acetyltransferase	-
EAF06_RS17205	3600682..3600888	+	207	Protein_3341	RtcB family protein	-
EAF06_RS17210	3600884..3601405	+	522	Protein_3342	peptide chain release factor H	-
EAF06_RS17215	3601462..3602919	-	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
EAF06_RS17220	3603180..3603638	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	3604234..3604314	+	81	NuclAT_11	-	-
-	3604234..3604314	+	81	NuclAT_11	-	-
-	3604234..3604314	+	81	NuclAT_11	-	-
-	3604234..3604314	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catA1 / blaOXA-1 / ant(3'')-Ia	gmhA/lpcA	3584838..3656134	71296

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T113273 WP_001263489.1 NZ_CP033158:3599504-3599902 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T113273 NZ_CP033158:3599504-3599902 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT113273 WP_000554758.1 NZ_CP033158:3599208-3599501 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT113273 NZ_CP033158:3599208-3599501 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3