Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 214245..214843 Replicon chromosome
Accession NZ_CP033144
Organism Vibrio owensii strain V180403

Toxin (Protein)


Gene name higB Uniprot ID A0A7Z7VLH5
Locus tag D0856_RS01005 Protein ID WP_054103799.1
Coordinates 214520..214843 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7Z7VJV6
Locus tag D0856_RS01000 Protein ID WP_054103798.1
Coordinates 214245..214523 (-) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D0856_RS00985 211207..213027 + 1821 WP_122067047.1 conjugative transfer system coupling protein TraD -
D0856_RS00990 213037..213597 + 561 WP_122067048.1 conjugative transfer protein -
D0856_RS00995 213584..214219 + 636 WP_029627967.1 DUF4400 domain-containing protein -
D0856_RS01000 214245..214523 - 279 WP_054103798.1 helix-turn-helix domain-containing protein Antitoxin
D0856_RS01005 214520..214843 - 324 WP_054103799.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D0856_RS01010 215024..215305 + 282 WP_000433889.1 type IV conjugative transfer system protein TraL -
D0856_RS01015 215302..215928 + 627 WP_000667170.1 hypothetical protein -
D0856_RS01020 215912..216808 + 897 WP_086597854.1 type-F conjugative transfer system secretin TraK -
D0856_RS01025 216811..218100 + 1290 WP_086597841.1 TraB/VirB10 family protein -
D0856_RS01030 218175..218747 + 573 WP_001944091.1 type IV conjugative transfer system lipoprotein TraV -
D0856_RS01035 218744..219130 + 387 WP_023584042.1 hypothetical protein -
D0856_RS01040 219171..219677 - 507 WP_023584043.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(15-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-81)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12295.35 Da        Isoelectric Point: 9.7658

>T113246 WP_054103799.1 NZ_CP033144:c214843-214520 [Vibrio owensii]
MSWKIDFYDGVEEQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Download         Length: 324 bp

>T113246 NZ_CP033144:c214843-214520 [Vibrio owensii]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGAGCAGATCCTCGATATGCCTCCAAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATTGGTAGAGGCTTATTTTGCTACTTAAAAGGGAAGCACATTTATGTGTTACAC
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTTAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10503.05 Da        Isoelectric Point: 9.9393

>AT113246 WP_054103798.1 NZ_CP033144:c214523-214245 [Vibrio owensii]
MSLQKLKQRALANADVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Download         Length: 279 bp

>AT113246 NZ_CP033144:c214523-214245 [Vibrio owensii]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGATGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGACCAATTGCTTACCATGAGAACAAAGGCAGGACTTACTCAAGAACAAGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGTAATGCCAATCCAAGTTGGTCAACTCTTCTAAAATATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z7VLH5

Antitoxin

Source ID Structure
AlphaFold DB A0A7Z7VJV6

References