Detailed information of TA system TA113234

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 2030299..2030932
• Replicon: chromosome
• Accession: NZ_CP033134
• Organism: Vibrio campbellii strain 170502

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A347UQS4
• Locus tag: D0784_RS09395
• Protein ID: WP_012600340.1
• Coordinates: 2030600..2030932 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D0784_RS09390
• Protein ID: WP_017049537.1
• Coordinates: 2030299..2030613 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D0784_RS09340	2025627..2025719	-	93	WP_206384574.1	DUF3265 domain-containing protein	-
D0784_RS09345	2025743..2026447	-	705	WP_122019774.1	hypothetical protein	-
D0784_RS09350	2026687..2027118	-	432	WP_122019775.1	GNAT family N-acetyltransferase	-
D0784_RS09355	2027217..2027306	-	90	WP_072606010.1	DUF3265 domain-containing protein	-
D0784_RS09360	2027837..2027929	-	93	WP_077681847.1	DUF3265 domain-containing protein	-
D0784_RS09365	2028384..2028476	-	93	WP_196298091.1	DUF3265 domain-containing protein	-
D0784_RS09370	2028591..2029559	+	969	WP_076611895.1	IS30-like element ISVa6 family transposase	-
D0784_RS09375	2029645..2029893	+	249	WP_005377003.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D0784_RS09380	2029883..2030173	+	291	WP_047010341.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D0784_RS09385	2030192..2030284	-	93	WP_080102040.1	DUF3265 domain-containing protein	-
D0784_RS09390	2030299..2030613	-	315	WP_017049537.1	DNA-binding transcriptional regulator	Antitoxin
D0784_RS09395	2030600..2030932	-	333	WP_012600340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D0784_RS09400	2031035..2031127	-	93	Protein_1780	DUF3265 domain-containing protein	-
D0784_RS09405	2031157..2031798	-	642	WP_122019776.1	hypothetical protein	-
D0784_RS09415	2032858..2032990	-	133	Protein_1782	DUF3265 domain-containing protein	-
D0784_RS09425	2033479..2033571	-	93	WP_193244088.1	DUF3265 domain-containing protein	-
D0784_RS09430	2033598..2034506	-	909	WP_122019778.1	phosphotransferase	-
D0784_RS09435	2034740..2034982	+	243	WP_122019779.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D0784_RS09440	2034990..2035289	+	300	WP_122019780.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D0784_RS09445	2035353..2035445	-	93	WP_079765077.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1992757..2070280	77523
	-	flank	IS/Tn	-	-	2028783..2029559	776
	-	inside	Integron	-	-	2003355..2070280	66925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Antitoxin MqsA/HigA-2 (24-101)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12906.76 Da
• Isoelectric Point: 10.0960

>T113234 WP_012600340.1 NZ_CP033134:c2030932-2030600 [Vibrio campbellii]
MKSVFVESTIFEKYRNEYLSDDEFRLFQAELMSNPKQGDVIQGTGGLRKIRVASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T113234 NZ_CP033134:c2030932-2030600 [Vibrio campbellii]
ATGAAAAGTGTATTTGTCGAATCAACCATATTTGAAAAGTACCGTAATGAATATCTCAGCGATGATGAGTTTAGGCTTTT
TCAAGCTGAGTTAATGTCTAACCCGAAGCAGGGGGACGTGATTCAAGGTACAGGTGGTCTGCGAAAGATTCGAGTTGCAA
GTAAAGGTAAGGGCAAGCGTGGTGGCTCTCGTGTTATCTATTACTTCCTCGATGAGAAGCGTCGTTTCTACTTGCTGACG
ATTTACGGCAAGAATGAAATGTCTGACTTAACTGCAGACCAAAAGAAACAACTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11704.17 Da
• Isoelectric Point: 6.3290

>AT113234 WP_017049537.1 NZ_CP033134:c2030613-2030299 [Vibrio campbellii]
MSNRDLFAELSSALVEAKEHSEGKLTLKTHQVNDISELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT113234 NZ_CP033134:c2030613-2030299 [Vibrio campbellii]
ATGAGCAATCGTGATCTATTTGCGGAGTTAAGTTCAGCACTTGTTGAGGCTAAAGAGCATTCAGAGGGCAAGCTTACCCT
TAAGACTCATCAAGTTAACGACATCAGTGAGTTAAATATCTCACCAGATGAAATCGTGAGTATTCGAGAGCAATTTAATA
TGTCTCGTGGTGTTTTTGCTCGTTTGTTGCACACATCATCTCGTACGTTAGAAAACTGGGAGCAAGGGCGTAGCGCACCA
AATGGACAAGCAGTAACCCTTCTAAAGTTGGTTCAGCGTCACCCAGAAACCCTGTCGCACATCGCTGAGCTATAA

Structures

Toxin

• AlphaFold DB: A0A347UQS4