Detailed information of TA system TA113198

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2134637..2135060
• Replicon: chromosome
• Accession: NZ_CP033112
• Organism: Staphylococcus aureus strain ST20130944

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5S9I4Y6
• Locus tag: SAST44_RS10540
• Protein ID: WP_001065091.1
• Coordinates: 2134809..2135060 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: SAST44_RS10535
• Protein ID: WP_000028422.1
• Coordinates: 2134637..2134819 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAST44_RS10490	2130023..2131684	-	1662	WP_000625088.1	terminase large subunit	-
SAST44_RS10495	2131681..2132025	-	345	WP_000402904.1	hypothetical protein	-
SAST44_RS10500	2132155..2132454	-	300	WP_000988336.1	HNH endonuclease	-
SAST44_RS10505	2132686..2133102	-	417	WP_000590122.1	hypothetical protein	-
SAST44_RS10510	2133130..2133330	-	201	WP_000265043.1	DUF1514 family protein	-
SAST44_RS10515	2133330..2133479	-	150	WP_000595265.1	transcriptional activator RinB	-
SAST44_RS10520	2133476..2133862	-	387	WP_000592207.1	hypothetical protein	-
SAST44_RS10525	2133859..2134065	-	207	WP_000195803.1	DUF1381 domain-containing protein	-
SAST44_RS10530	2134102..2134644	-	543	WP_000185659.1	dUTP pyrophosphatase	-
SAST44_RS10535	2134637..2134819	-	183	WP_000028422.1	hypothetical protein	Antitoxin
SAST44_RS10540	2134809..2135060	-	252	WP_001065091.1	DUF1024 family protein	Toxin
SAST44_RS14480	2135053..2135217	-	165	WP_000990744.1	hypothetical protein	-
SAST44_RS10545	2135232..2135474	-	243	WP_000131366.1	hypothetical protein	-
SAST44_RS10550	2135478..2135846	-	369	WP_000101274.1	hypothetical protein	-
SAST44_RS10555	2135859..2136168	-	310	Protein_2041	RusA family crossover junction endodeoxyribonuclease	-
SAST44_RS10560	2136177..2136395	-	219	WP_000338531.1	hypothetical protein	-
SAST44_RS10565	2136402..2137286	-	885	WP_000148316.1	DnaD domain protein	-
SAST44_RS10570	2137316..2137786	-	471	WP_000934764.1	single-stranded DNA-binding protein	-
SAST44_RS10575	2137787..2138404	-	618	WP_071621397.1	MBL fold metallo-hydrolase	-
SAST44_RS10580	2138485..2139405	-	921	WP_000138472.1	recombinase RecT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / chp / sak / sea / hlb / tsst-1 / groEL	2106969..2174933	67964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9349.22 Da
• Isoelectric Point: 3.9473

>T113198 WP_001065091.1 NZ_CP033112:c2135060-2134809 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T113198 NZ_CP033112:c2135060-2134809 [Staphylococcus aureus]
ATGAATAATCGCGAACAAATTGAACAATCAGTTATAAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTACTAAA
AGAGATTGAGGACGTTTATAAGAAAGCGCAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTGTTGAACTTGATGAAGCAGTAGGGATTATGGCAGGTCAAGTTGTCTATAAATATGAGGAGGAACAGGAA
AATGAGCATTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT113198 WP_000028422.1 NZ_CP033112:c2134819-2134637 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT113198 NZ_CP033112:c2134819-2134637 [Staphylococcus aureus]
ATGAGCATTAGTGTAGGAGACAAGGTTTTTAATCCAGAAACAAATTCAACTTTAGAAATTGTACAACTTGTTGGCGATAT
TAGAGACACGCATTACAAGTTATCTGACGGATCTATTATTAGTCTTATAGACTTTGTTGTTAAACCAATTCATTTAATCA
AGGAGGAGCAGGAAAATGACTAA

Structures

Toxin

• AlphaFold DB: A0A5S9I4Y6

Antitoxin

• AlphaFold DB: A0A0E0VQM8