Detailed information of TA system TA113141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 28754..29484
• Replicon: chromosome
• Accession: NZ_CP033102
• Organism: Enterobacter hormaechei strain L51

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155SSD8
• Locus tag: D8768_RS00145
• Protein ID: WP_022646552.1
• Coordinates: 28754..29068 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D8768_RS00150
• Protein ID: WP_022646553.1
• Coordinates: 29065..29484 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8768_RS00120	24835..25920	+	1086	Protein_23	cellulase family glycosylhydrolase	-
D8768_RS00125	25826..27010	-	1185	WP_157506406.1	multidrug efflux MFS transporter EmrD	-
D8768_RS00130	27186..27604	-	419	Protein_25	EamA family transporter	-
D8768_RS00135	27667..28113	-	447	WP_157506408.1	GNAT family N-acetyltransferase	-
D8768_RS00140	28178..28267	-	90	WP_022646551.1	type I toxin-antitoxin system toxin TisB	-
D8768_RS00145	28754..29068	+	315	WP_022646552.1	type II toxin-antitoxin system HigB family toxin	Toxin
D8768_RS00150	29065..29484	+	420	WP_022646553.1	helix-turn-helix domain-containing protein	Antitoxin
D8768_RS00155	29631..29729	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
D8768_RS00160	29836..31524	+	1689	WP_157506410.1	acetolactate synthase large subunit	-
D8768_RS00165	31528..31815	+	288	WP_045336030.1	acetolactate synthase small subunit	-
D8768_RS00170	31941..32534	+	594	WP_022646555.1	transcriptional regulator UhpA	-
D8768_RS00175	32531..34036	+	1506	WP_157506411.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Antitoxin HigA (1-139)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12441.55 Da
• Isoelectric Point: 10.1989

>T113141 WP_022646552.1 NZ_CP033102:28754-29068 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQYREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T113141 NZ_CP033102:28754-29068 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAATACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15480.10 Da
• Isoelectric Point: 5.6761

>AT113141 WP_022646553.1 NZ_CP033102:29065-29484 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANRPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT113141 NZ_CP033102:29065-29484 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGCCCCTTGCTGGATATTGTTT
GCGCCAGAATTAGCCATTACGAAGCTAATCGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTGAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGCTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A155SSD8