Detailed information of TA system TA113128

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3640912..3641606
• Replicon: chromosome
• Accession: NZ_CP033096
• Organism: Escherichia coli strain CP53

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: EAH50_RS20065
• Protein ID: WP_001263493.1
• Coordinates: 3640912..3641310 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: EAH50_RS20070
• Protein ID: WP_000554757.1
• Coordinates: 3641313..3641606 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAH50_RS20035	3635912..3637156	-	1245	WP_000189541.1	esterase FrsA	-
-	3636572..3636652	-	81	NuclAT_9	-	-
-	3636572..3636652	-	81	NuclAT_9	-	-
-	3636572..3636652	-	81	NuclAT_9	-	-
-	3636572..3636652	-	81	NuclAT_9	-	-
EAH50_RS20040	3637248..3637706	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
EAH50_RS20045	3637967..3639424	+	1458	WP_122057011.1	cytosol nonspecific dipeptidase	-
EAH50_RS20050	3639481..3640002	-	522	Protein_3460	peptide chain release factor H	-
EAH50_RS20055	3640001..3640204	-	204	Protein_3461	RNA ligase RtcB family protein	-
EAH50_RS20060	3640450..3640902	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
EAH50_RS20065	3640912..3641310	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EAH50_RS20070	3641313..3641606	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EAH50_RS20075	3641658..3642713	-	1056	WP_001226164.1	DNA polymerase IV	-
EAH50_RS20080	3642784..3643569	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
EAH50_RS20085	3643541..3645253	+	1713	Protein_3467	flagellar biosynthesis protein FlhA	-
EAH50_RS20090	3645477..3645974	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T113128 WP_001263493.1 NZ_CP033096:c3641310-3640912 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T113128 NZ_CP033096:c3641310-3640912 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT113128 WP_000554757.1 NZ_CP033096:c3641606-3641313 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT113128 NZ_CP033096:c3641606-3641313 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6