Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1782773..1783275 Replicon chromosome
Accession NZ_CP033096
Organism Escherichia coli strain CP53

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EAH50_RS10720 Protein ID WP_000767829.1
Coordinates 1783021..1783275 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EAH50_RS10715 Protein ID WP_001259255.1
Coordinates 1782773..1783024 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EAH50_RS10690 1777951..1779018 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EAH50_RS10695 1779018..1780088 - 1071 WP_000108940.1 histidinol-phosphate transaminase -
EAH50_RS10700 1780085..1781389 - 1305 WP_029402287.1 histidinol dehydrogenase -
EAH50_RS10705 1781395..1782294 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EAH50_RS10710 1782440..1782490 - 51 WP_001364200.1 his operon leader peptide -
EAH50_RS10715 1782773..1783024 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EAH50_RS10720 1783021..1783275 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EAH50_RS10725 1783358..1784182 + 825 WP_000754758.1 SDR family oxidoreductase -
EAH50_RS10730 1784228..1785157 + 930 WP_000803351.1 LysR family transcriptional regulator -
EAH50_RS10735 1785372..1785434 + 63 WP_010723108.1 membrane protein YoeI -
EAH50_RS10740 1785424..1786782 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EAH50_RS10745 1786961..1788019 + 1059 WP_000492340.1 transport protein YeeE -
EAH50_RS10750 1788033..1788260 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T113116 WP_000767829.1 NZ_CP033096:1783021-1783275 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T113116 NZ_CP033096:1783021-1783275 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT113116 WP_001259255.1 NZ_CP033096:1782773-1783024 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT113116 NZ_CP033096:1782773-1783024 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References