Detailed information of TA system TA113103

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4073389..4073798
• Replicon: chromosome
• Accession: NZ_CP033092
• Organism: Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A0E2KY59
• Locus tag: EAS44_RS20950
• Protein ID: WP_000132639.1
• Coordinates: 4073460..4073798 (+)
• Length: 113 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4073389..4073465 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EAS44_RS20940	4068496..4069887	+	1392	WP_000535334.1	restriction endonuclease subunit S	-
EAS44_RS20945	4069982..4073245	+	3264	WP_000058888.1	type I restriction endonuclease subunit R	-
-	4073389..4073465	-	77	NuclAT_24	-	Antitoxin
-	4073389..4073465	-	77	NuclAT_24	-	Antitoxin
-	4073389..4073465	-	77	NuclAT_24	-	Antitoxin
-	4073389..4073465	-	77	NuclAT_24	-	Antitoxin
-	4073389..4073465	-	77	NuclAT_25	-	Antitoxin
-	4073389..4073465	-	77	NuclAT_25	-	Antitoxin
-	4073389..4073465	-	77	NuclAT_25	-	Antitoxin
-	4073389..4073465	-	77	NuclAT_25	-	Antitoxin
EAS44_RS20950	4073460..4073798	+	339	WP_000132639.1	endoribonuclease SymE	Toxin
EAS44_RS20955	4073840..4074004	-	165	WP_000394277.1	DUF1127 domain-containing protein	-
EAS44_RS20960	4074181..4075593	+	1413	WP_000199313.1	PLP-dependent aminotransferase family protein	-
EAS44_RS20965	4075836..4076780	-	945	WP_000181173.1	Rpn family recombination-promoting nuclease/putative transposase	-
EAS44_RS20980	4077247..4078479	+	1233	WP_001137021.1	multidrug efflux MFS transporter MdtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12308.07 Da
• Isoelectric Point: 7.8234

>T113103 WP_000132639.1 NZ_CP033092:4073460-4073798 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
MTDTHSIAQPFEPEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAEEGE
LMQSLRQVCKLSARKQRQVQDFIGVISSKTPR

Nucleotide

• Length: 339 bp

>T113103 NZ_CP033092:4073460-4073798 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAACCAGAAGTCTCTCCTGCAAATAACCGTCAATTAACCGTGAGCTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGTGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGGGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAGGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAGCAAAACGCCACGCTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT113103 NZ_CP033092:c4073465-4073389 [Escherichia coli DSM 30083 = JCM 1649 = ATCC 11775]
AGTCATGATTACTATTCTCCATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E2KY59

Antitoxin