Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4799007..4799673 Replicon chromosome
Accession NZ_CP033076
Organism Buttiauxella sp. 3AFRM03

Toxin (Protein)


Gene name higB Uniprot ID A0A3G2IJL7
Locus tag D8682_RS23855 Protein ID WP_064545177.1
Coordinates 4799007..4799354 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A3G2IKP2
Locus tag D8682_RS23860 Protein ID WP_064545179.1
Coordinates 4799356..4799673 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D8682_RS23845 4794402..4797527 - 3126 WP_121815290.1 MdtB/MuxB family multidrug efflux RND transporter permease subunit -
D8682_RS23850 4797527..4798777 - 1251 WP_121815291.1 MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit -
D8682_RS23855 4799007..4799354 + 348 WP_064545177.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
D8682_RS23860 4799356..4799673 + 318 WP_064545179.1 helix-turn-helix transcriptional regulator Antitoxin
D8682_RS23865 4799705..4801063 - 1359 WP_064545181.1 molecular chaperone -
D8682_RS23870 4801161..4802012 + 852 WP_121815292.1 DNA-3-methyladenine glycosylase 2 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13795.97 Da        Isoelectric Point: 9.8806

>T113067 WP_064545177.1 NZ_CP033076:4799007-4799354 [Buttiauxella sp. 3AFRM03]
MTWKILTTKRYEEWFDEQHNDLQEKMLAVMGHLRFWGPCLPRPYADTVNGSRFANMKELRVQHQGKPVRAFYIFDYRRNA
VLLCAGDKSKTKRFYESMITIADKEFIAWLKSQEK

Download         Length: 348 bp

>T113067 NZ_CP033076:4799007-4799354 [Buttiauxella sp. 3AFRM03]
ATGACATGGAAGATATTGACTACAAAACGGTATGAAGAATGGTTTGATGAGCAACACAATGATCTTCAGGAAAAGATGCT
TGCAGTGATGGGGCATTTACGGTTTTGGGGGCCTTGTTTGCCTCGTCCCTATGCCGACACTGTAAATGGTTCACGTTTTG
CCAACATGAAAGAGTTGAGAGTTCAGCACCAGGGTAAGCCCGTTCGGGCATTTTATATTTTTGATTACCGCCGGAATGCT
GTTCTGTTGTGCGCAGGTGATAAGTCGAAAACTAAACGGTTCTACGAATCGATGATCACGATTGCGGACAAAGAGTTTAT
AGCTTGGTTGAAGTCACAGGAAAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11766.59 Da        Isoelectric Point: 8.0036

>AT113067 WP_064545179.1 NZ_CP033076:4799356-4799673 [Buttiauxella sp. 3AFRM03]
MKNNPKGYSTLEELIARRSPESQQRIAEATQELILESCLNMIREQMGWSQQQLADAMGISQPAVTAIEKRGNEVKLGTLK
RYIEALGGKLSLQIEFPDGVKHLKI

Download         Length: 318 bp

>AT113067 NZ_CP033076:4799356-4799673 [Buttiauxella sp. 3AFRM03]
ATGAAAAACAATCCGAAAGGTTACAGCACATTAGAAGAGTTAATTGCTCGTCGTTCACCTGAAAGTCAGCAACGAATAGC
TGAAGCGACTCAGGAACTGATTCTGGAAAGCTGTTTGAATATGATTCGCGAGCAAATGGGCTGGTCGCAGCAACAACTGG
CCGACGCGATGGGCATTAGCCAACCGGCGGTGACGGCGATTGAAAAACGCGGTAATGAAGTCAAATTGGGAACGTTAAAA
CGTTATATCGAAGCGTTGGGCGGCAAGCTTTCGCTGCAAATTGAATTTCCCGATGGGGTGAAACATCTGAAAATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G2IJL7

Antitoxin

Source ID Structure
AlphaFold DB A0A3G2IKP2

References