Detailed information of TA system TA113061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1772187..1772716
• Replicon: chromosome
• Accession: NZ_CP033076
• Organism: Buttiauxella sp. 3AFRM03

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D8682_RS09805
• Protein ID: WP_121814166.1
• Coordinates: 1772187..1772354 (+)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A3G2ICS7
• Locus tag: D8682_RS09810
• Protein ID: WP_064548926.1
• Coordinates: 1772387..1772716 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8682_RS09785	1767974..1769206	-	1233	WP_121814163.1	enterobactin transporter EntS	-
D8682_RS09790	1769348..1770337	+	990	WP_121814164.1	Fe(3+)-siderophore ABC transporter permease	-
D8682_RS09795	1770334..1771326	+	993	WP_064548922.1	iron-enterobactin ABC transporter permease	-
D8682_RS09800	1771323..1772123	+	801	WP_121814165.1	iron-enterobactin ABC transporter ATP-binding protein	-
D8682_RS09805	1772187..1772354	+	168	WP_121814166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D8682_RS09810	1772387..1772716	+	330	WP_064548926.1	HigA family addiction module antidote protein	Antitoxin
D8682_RS09815	1772756..1776649	-	3894	WP_121815677.1	enterobactin synthase subunit F	-
D8682_RS09820	1776657..1776878	-	222	WP_121814167.1	MbtH family NRPS accessory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (19-49)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6501.36 Da
• Isoelectric Point: 9.5733

>T113061 WP_121814166.1 NZ_CP033076:1772187-1772354 [Buttiauxella sp. 3AFRM03]
MQGMLYCPSGHSDGTSIMEKYSSIRVNNQFRLIFIWRNGKAHNIYLDDHGYRKHN

Nucleotide

• Length: 168 bp

>T113061 NZ_CP033076:1772187-1772354 [Buttiauxella sp. 3AFRM03]
ATGCAAGGTATGCTCTACTGCCCATCCGGTCACTCAGATGGAACCAGCATCATGGAGAAGTACTCCTCTATAAGAGTGAA
CAACCAGTTCCGGCTAATCTTCATCTGGCGTAACGGCAAAGCGCACAATATTTACCTTGATGATCATGGCTATCGAAAAC
ATAACTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 12649.55 Da
• Isoelectric Point: 7.0138

>AT113061 WP_064548926.1 NZ_CP033076:1772387-1772716 [Buttiauxella sp. 3AFRM03]
MVQAQRKPTTVGDVLLYEYLEPTGLRINDLAEMLQVHRNTVSKLLNNNSKLTTDMAFRLAKAFDTSVDFWLNLQTNLDVW
EIQNDPRTQVEISRIITLKEMLERKTRAA

Nucleotide

• Length: 330 bp

>AT113061 NZ_CP033076:1772387-1772716 [Buttiauxella sp. 3AFRM03]
ATGGTCCAGGCACAGCGTAAACCGACCACCGTTGGTGACGTGCTGCTTTATGAATACCTCGAACCGACTGGCTTGCGTAT
TAACGATCTGGCCGAAATGCTTCAGGTACATCGAAATACGGTGAGTAAATTGCTCAATAACAACAGTAAACTCACCACTG
ATATGGCCTTCAGACTGGCAAAAGCATTTGATACTTCTGTAGATTTTTGGCTGAATCTACAAACCAATCTCGATGTCTGG
GAAATCCAAAACGACCCACGTACCCAGGTAGAAATTAGCCGTATCATCACGCTCAAAGAGATGCTTGAGCGTAAAACTCG
CGCGGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A3G2ICS7