Detailed information of TA system TA113050

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 425307..426661
• Replicon: chromosome
• Accession: NZ_CP033076
• Organism: Buttiauxella sp. 3AFRM03

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: -
• Locus tag: D8682_RS03185
• Protein ID: WP_121813521.1
• Coordinates: 425624..426661 (+)
• Length: 346 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: A0A3G2I9X5
• Locus tag: D8682_RS03180
• Protein ID: WP_064546608.1
• Coordinates: 425307..425621 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8682_RS03160	421151..422461	-	1311	WP_121813519.1	LLM class flavin-dependent oxidoreductase	-
D8682_RS03165	422525..423646	-	1122	WP_064546614.1	amidohydrolase	-
D8682_RS03170	423657..424685	-	1029	WP_121813520.1	MsnO8 family LLM class oxidoreductase	-
D8682_RS03175	424999..425322	+	324	WP_064546610.1	helix-turn-helix domain-containing protein	-
D8682_RS03180	425307..425621	+	315	WP_064546608.1	HipA N-terminal domain-containing protein	Antitoxin
D8682_RS03185	425624..426661	+	1038	WP_121813521.1	HipA domain-containing protein	Toxin
D8682_RS03190	426711..428375	-	1665	WP_121813522.1	methyl-accepting chemotaxis protein	-
D8682_RS03195	428462..429046	-	585	WP_083963375.1	PadR family transcriptional regulator	-
D8682_RS03200	429057..429818	-	762	WP_121813523.1	siderophore-interacting protein	-
D8682_RS03205	430089..430304	+	216	WP_064546600.1	YdcH family protein	-
D8682_RS03210	430345..430662	-	318	WP_121813524.1	N(4)-acetylcytidine aminohydrolase	-
D8682_RS03215	430703..430876	-	174	WP_064546596.1	GhoT/OrtT family toxin	-
D8682_RS03220	431043..431231	+	189	WP_064546594.1	YegP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-304)

Antitoxin

• HipA, N-terminal subdomain 1 (5-99)

Sequences

Toxin

Protein

• Length: 346 a.a.
• Molecular weight: 38941.61 Da
• Isoelectric Point: 6.4312

>T113050 WP_121813521.1 NZ_CP033076:425624-426661 [Buttiauxella sp. 3AFRM03]
MRCHILLTPLLQAKEQESGYSSKGLKQLTGSGKVNPQLSFTRDDFIHELPKAQRGMSISGYQPKLQMVVRNGEFAVVEHQ
GEYILKPSPAEFPHLAENEHATMTLMARLGFDVPPHGLVSFAAVDNEAERELAFVIRRFDRDIQTGAAIHQEQLDAAMNV
GEKYGKTGSDGKQYVSYQQLAEFLTQHVNSNIAFKIDLFRRIAYAYLLGNNDMHLRNFGLIHPRIGEPTLSPVYDFVSVA
PYPAIFSSGFMALPLLRCEEGDRELAPGFKTAYGEYLGMDFQALGLSMGMTEKLIVKLLNDMRKESRIVDAVYRDSFMPA
KDVEATLRCYHQRLDRLMRLDEAEL

Nucleotide

• Length: 1038 bp

>T113050 NZ_CP033076:425624-426661 [Buttiauxella sp. 3AFRM03]
ATGCGTTGCCACATTCTTTTAACTCCATTGCTCCAGGCCAAAGAGCAAGAGTCGGGCTATTCATCCAAAGGCCTTAAACA
GCTGACGGGTTCGGGCAAGGTGAACCCGCAACTGAGTTTTACCCGTGATGACTTTATTCATGAATTGCCGAAAGCGCAGC
GTGGCATGAGTATTTCCGGCTACCAGCCGAAACTGCAAATGGTGGTGCGTAACGGTGAATTTGCGGTGGTTGAACATCAG
GGCGAATACATCCTCAAGCCTTCTCCGGCGGAATTCCCCCATCTGGCAGAAAACGAACACGCCACCATGACGTTAATGGC
GCGACTGGGGTTTGATGTGCCGCCACACGGGCTGGTGAGCTTTGCCGCTGTTGATAACGAAGCGGAGCGCGAGTTGGCAT
TTGTTATCCGTCGTTTCGACCGGGATATTCAAACCGGAGCGGCCATACATCAGGAACAGCTTGATGCAGCGATGAACGTC
GGCGAAAAGTACGGGAAGACGGGCAGCGACGGAAAGCAGTATGTCAGTTATCAGCAACTGGCCGAGTTCCTGACACAACA
TGTGAATAGCAACATCGCCTTTAAAATCGATTTGTTCCGGCGCATTGCCTACGCGTATTTGTTGGGGAATAACGATATGC
ACTTGCGCAATTTCGGGCTGATTCATCCACGTATTGGCGAACCAACGCTGTCGCCAGTGTATGACTTTGTGTCGGTTGCG
CCCTATCCCGCCATTTTTAGCTCCGGTTTTATGGCGCTGCCGCTATTAAGATGTGAAGAAGGCGACCGCGAATTAGCGCC
AGGTTTTAAGACCGCTTACGGCGAATATCTTGGTATGGATTTTCAGGCGTTGGGTTTGAGTATGGGGATGACGGAGAAAC
TTATTGTTAAATTGCTCAACGATATGCGCAAAGAAAGCCGCATCGTAGATGCCGTTTATCGAGATTCGTTTATGCCCGCA
AAAGACGTGGAAGCGACGTTGCGTTGTTACCACCAGCGCCTGGATCGTTTAATGCGCTTAGATGAAGCGGAGTTGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12014.68 Da
• Isoelectric Point: 6.2228

>AT113050 WP_064546608.1 NZ_CP033076:425307-425621 [Buttiauxella sp. 3AFRM03]
MRRRVEVWLYGEPVGELRQHDDGFEFSYRADYIGPPLSLSLPVRVGHFRSQTLPPFFASLAPEGWLKKRYSQLQQRDEHD
LLGMLIDNGKNLIGAVQLVSMQED

Nucleotide

• Length: 315 bp

>AT113050 NZ_CP033076:425307-425621 [Buttiauxella sp. 3AFRM03]
ATGCGCCGCCGTGTAGAGGTGTGGTTATATGGCGAACCGGTCGGCGAACTGCGCCAGCATGACGACGGCTTCGAATTTAG
TTATCGCGCTGACTACATCGGCCCGCCGCTGTCGCTAAGTTTGCCGGTGCGTGTCGGCCACTTTCGCAGCCAGACACTGC
CGCCCTTTTTTGCCTCGCTGGCACCAGAAGGCTGGCTGAAAAAGCGCTACAGCCAACTACAACAACGCGACGAGCACGAC
CTGCTCGGAATGCTTATCGATAACGGCAAAAACCTGATTGGCGCGGTACAGCTGGTCAGCATGCAGGAGGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A3G2I9X5