Detailed information of TA system TA113016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4367163..4367725
• Replicon: chromosome
• Accession: NZ_CP033055
• Organism: Serratia sp. 3ACOL1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3G2FJW6
• Locus tag: D9980_RS19720
• Protein ID: WP_024483282.1
• Coordinates: 4367447..4367725 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D9980_RS19715
• Protein ID: WP_121608220.1
• Coordinates: 4367163..4367447 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9980_RS19700	4362747..4363319	-	573	WP_161629419.1	MltR family transcriptional regulator	-
D9980_RS19705	4363469..4364617	-	1149	WP_121608219.1	mannitol-1-phosphate 5-dehydrogenase	-
D9980_RS19710	4364680..4366596	-	1917	WP_024483280.1	PTS mannitol transporter subunit IICBA	-
D9980_RS19715	4367163..4367447	-	285	WP_121608220.1	HigA family addiction module antidote protein	Antitoxin
D9980_RS19720	4367447..4367725	-	279	WP_024483282.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9980_RS19725	4367994..4368713	-	720	WP_024483283.1	DUF3053 domain-containing protein	-
D9980_RS19730	4368911..4370980	-	2070	WP_024483284.1	glycine--tRNA ligase subunit beta	-
D9980_RS19735	4370989..4371897	-	909	WP_024483285.1	glycine--tRNA ligase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10486.86 Da
• Isoelectric Point: 9.4604

>T113016 WP_024483282.1 NZ_CP033055:c4367725-4367447 [Serratia sp. 3ACOL1]
MIKSFKHKGLRQFFEKGSTAGINTQQIGKIRLRLGVIDAAKDISDINRPGYQLHPLSGDRAGQWSITVTGNWRITFEFID
GDAYIVNYEDYH

Nucleotide

• Length: 279 bp

>T113016 NZ_CP033055:c4367725-4367447 [Serratia sp. 3ACOL1]
ATGATTAAGAGTTTTAAGCACAAGGGATTGCGACAGTTTTTTGAGAAAGGCTCTACGGCTGGCATCAATACTCAACAGAT
CGGTAAAATTCGTCTGAGGCTTGGTGTAATTGATGCAGCAAAGGACATCAGTGATATCAACCGCCCTGGCTATCAACTTC
ACCCATTGTCGGGTGACCGTGCCGGGCAGTGGTCAATAACCGTAACAGGCAACTGGCGCATCACGTTTGAATTTATCGAC
GGTGATGCTTATATCGTCAACTATGAGGATTATCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10108.82 Da
• Isoelectric Point: 7.2022

>AT113016 WP_121608220.1 NZ_CP033055:c4367447-4367163 [Serratia sp. 3ACOL1]
MAMHNPPHPGEIIAETLEELGVGIRVFARALMVAPSTAQRLVAGNAAVSPEMAVKLSIVLGSSPEFWLNLQDNYSLWIAK
KAVDTSQLKRLVAA

Nucleotide

• Length: 285 bp

>AT113016 NZ_CP033055:c4367447-4367163 [Serratia sp. 3ACOL1]
ATGGCTATGCATAACCCCCCGCATCCGGGCGAGATCATCGCTGAAACGCTGGAAGAACTGGGTGTTGGTATCCGCGTATT
TGCCAGGGCGTTGATGGTGGCACCCTCCACCGCACAGCGCTTGGTCGCAGGGAATGCGGCTGTTAGCCCTGAGATGGCGG
TGAAGTTGTCCATAGTGCTGGGCAGTTCCCCTGAATTCTGGCTAAACCTGCAGGATAATTACAGCCTGTGGATCGCCAAA
AAAGCAGTCGATACTTCGCAGCTAAAGCGTTTGGTTGCCGCTTGA

Structures

Toxin

• AlphaFold DB: A0A3G2FJW6