Detailed information of TA system TA112973

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4067610..4068022
• Replicon: chromosome
• Accession: NZ_CP033020
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: symE
• Uniprot ID: B1IS82
• Locus tag: D9Q99_RS19605
• Protein ID: WP_000132625.1
• Coordinates: 4067681..4068022 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4067610..4067686 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9Q99_RS19595 (4065041)	4065041..4065703	+	663	Protein_3830	SAM-dependent methyltransferase	-
D9Q99_RS19600 (4065703)	4065703..4067460	+	1758	WP_000110077.1	restriction endonuclease subunit S	-
- (4067610)	4067610..4067686	-	77	NuclAT_12	-	Antitoxin
- (4067610)	4067610..4067686	-	77	NuclAT_12	-	Antitoxin
- (4067610)	4067610..4067686	-	77	NuclAT_12	-	Antitoxin
- (4067610)	4067610..4067686	-	77	NuclAT_12	-	Antitoxin
- (4067610)	4067610..4067686	-	77	NuclAT_13	-	Antitoxin
- (4067610)	4067610..4067686	-	77	NuclAT_13	-	Antitoxin
- (4067610)	4067610..4067686	-	77	NuclAT_13	-	Antitoxin
- (4067610)	4067610..4067686	-	77	NuclAT_13	-	Antitoxin
D9Q99_RS19605 (4067681)	4067681..4068022	+	342	WP_000132625.1	endoribonuclease SymE	Toxin
D9Q99_RS19610 (4068069)	4068069..4069232	-	1164	WP_225402390.1	DUF1524 domain-containing protein	-
D9Q99_RS19615 (4069280)	4069280..4070162	-	883	Protein_3834	DUF262 domain-containing protein	-
D9Q99_RS19620 (4070305)	4070305..4070457	-	153	WP_001496262.1	hypothetical protein	-
D9Q99_RS19625 (4070600)	4070600..4072012	+	1413	WP_000199353.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12270.10 Da
• Isoelectric Point: 8.4952

>T112973 WP_000132625.1 NZ_CP033020:4067681-4068022 [Escherichia coli ATCC 8739]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVVVKVMEGCIVLTAQPAAAEESE
LMQSLRQVCKLSARKQKQVQDFIGVITGKQKVA

Nucleotide

• Length: 342 bp

>T112973 NZ_CP033020:4067681-4068022 [Escherichia coli ATCC 8739]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGCCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGTTGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCCGCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTAC
AGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT112973 NZ_CP033020:c4067686-4067610 [Escherichia coli ATCC 8739]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: B1IS82

Antitoxin