Detailed information of TA system TA112955

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 725975..726668
• Replicon: chromosome
• Accession: NZ_CP033020
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: -
• Locus tag: D9Q99_RS03525
• Protein ID: WP_000415583.1
• Coordinates: 725975..726271 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: D9Q99_RS03530
• Protein ID: WP_000650107.1
• Coordinates: 726273..726668 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9Q99_RS03490 (721063)	721063..721377	-	315	WP_000958598.1	putative quinol monooxygenase	-
D9Q99_RS03495 (721408)	721408..721989	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
D9Q99_RS03500 (722308)	722308..722640	+	333	WP_000917684.1	DUF2645 family protein	-
D9Q99_RS03505 (722686)	722686..724035	-	1350	WP_000673401.1	quorum sensing histidine kinase QseC	-
D9Q99_RS03510 (724032)	724032..724691	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
D9Q99_RS03515 (724843)	724843..725235	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
D9Q99_RS03520 (725288)	725288..725770	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
D9Q99_RS03525 (725975)	725975..726271	+	297	WP_000415583.1	type II toxin-antitoxin system toxin MqsR	Toxin
D9Q99_RS03530 (726273)	726273..726668	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
D9Q99_RS03535 (726801)	726801..728408	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
D9Q99_RS03540 (728546)	728546..730804	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (2-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11216.99 Da
• Isoelectric Point: 8.9070

>T112955 WP_000415583.1 NZ_CP033020:725975-726271 [Escherichia coli ATCC 8739]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGLV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T112955 NZ_CP033020:725975-726271 [Escherichia coli ATCC 8739]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCTGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT112955 WP_000650107.1 NZ_CP033020:726273-726668 [Escherichia coli ATCC 8739]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT112955 NZ_CP033020:726273-726668 [Escherichia coli ATCC 8739]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LU65