Detailed information of TA system TA112954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 661406..662133
• Replicon: chromosome
• Accession: NZ_CP033020
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1EX98
• Locus tag: D9Q99_RS03220
• Protein ID: WP_000550192.1
• Coordinates: 661406..661720 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D9Q99_RS03225
• Protein ID: WP_000560266.1
• Coordinates: 661717..662133 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9Q99_RS03200 (657573)	657573..658559	-	987	WP_000617698.1	Gfo/Idh/MocA family oxidoreductase	-
D9Q99_RS03205 (658638)	658638..659321	-	684	WP_001183046.1	vancomycin high temperature exclusion protein	-
D9Q99_RS03210 (659398)	659398..659901	-	504	WP_001295542.1	M48 family metallopeptidase	-
D9Q99_RS03215 (659986)	659986..661122	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
D9Q99_RS03220 (661406)	661406..661720	+	315	WP_000550192.1	type II toxin-antitoxin system toxin HigB	Toxin
D9Q99_RS03225 (661717)	661717..662133	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
D9Q99_RS03230 (662178)	662178..664196	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
D9Q99_RS03235 (664622)	664622..666973	-	2352	WP_000695486.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.08 Da
• Isoelectric Point: 10.0409

>T112954 WP_000550192.1 NZ_CP033020:661406-661720 [Escherichia coli ATCC 8739]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T112954 NZ_CP033020:661406-661720 [Escherichia coli ATCC 8739]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCCTAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT112954 WP_000560266.1 NZ_CP033020:661717-662133 [Escherichia coli ATCC 8739]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT112954 NZ_CP033020:661717-662133 [Escherichia coli ATCC 8739]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: S1EX98