Detailed information of TA system TA112953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 612816..613615
• Replicon: chromosome
• Accession: NZ_CP033020
• Organism: Escherichia coli ATCC 8739

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: D9Q99_RS02975
• Protein ID: WP_000347251.1
• Coordinates: 612816..613280 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: D9Q99_RS02980
• Protein ID: WP_012304834.1
• Coordinates: 613280..613615 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9Q99_RS02945 (607817)	607817..608251	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
D9Q99_RS02950 (608269)	608269..609147	-	879	WP_012304833.1	PTS N-acetylgalactosamine transporter subunit IID	-
D9Q99_RS02955 (609137)	609137..609916	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
D9Q99_RS02960 (609927)	609927..610400	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
D9Q99_RS02965 (610423)	610423..611703	-	1281	WP_000681905.1	tagatose-bisphosphate aldolase subunit KbaZ	-
D9Q99_RS02970 (611952)	611952..612761	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
D9Q99_RS02975 (612816)	612816..613280	-	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
D9Q99_RS02980 (613280)	613280..613615	-	336	WP_012304834.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
D9Q99_RS02985 (613764)	613764..615335	-	1572	WP_001273760.1	galactarate dehydratase	-
D9Q99_RS02990 (615710)	615710..617044	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
D9Q99_RS02995 (617060)	617060..617830	+	771	WP_001058236.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T112953 WP_000347251.1 NZ_CP033020:c613280-612816 [Escherichia coli ATCC 8739]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T112953 NZ_CP033020:c613280-612816 [Escherichia coli ATCC 8739]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12344.95 Da
• Isoelectric Point: 4.6890

>AT112953 WP_012304834.1 NZ_CP033020:c613615-613280 [Escherichia coli ATCC 8739]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT112953 NZ_CP033020:c613615-613280 [Escherichia coli ATCC 8739]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCCGGCCTGGACAGCATTCATTACGAAATTCTCCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20