Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3566916..3567610 Replicon chromosome
Accession NZ_CP032992
Organism Escherichia coli strain W5-6

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag D9V54_RS17635 Protein ID WP_001263489.1
Coordinates 3566916..3567314 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag D9V54_RS17640 Protein ID WP_000554758.1
Coordinates 3567317..3567610 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3562504..3562584 - 81 NuclAT_12 - -
- 3562504..3562584 - 81 NuclAT_12 - -
- 3562504..3562584 - 81 NuclAT_12 - -
- 3562504..3562584 - 81 NuclAT_12 - -
D9V54_RS17610 3563180..3563638 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
D9V54_RS17615 3563899..3565356 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
D9V54_RS17620 3565413..3565934 - 522 Protein_3379 peptide chain release factor H -
D9V54_RS17625 3565930..3566136 - 207 Protein_3380 RtcB family protein -
D9V54_RS17630 3566454..3566906 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
D9V54_RS17635 3566916..3567314 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
D9V54_RS17640 3567317..3567610 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
D9V54_RS17645 3567662..3568717 - 1056 WP_001226164.1 DNA polymerase IV -
D9V54_RS17650 3568788..3569573 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
D9V54_RS17655 3569545..3571257 + 1713 Protein_3386 flagellar biosynthesis protein FlhA -
D9V54_RS17660 3571481..3571978 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T112941 WP_001263489.1 NZ_CP032992:c3567314-3566916 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T112941 NZ_CP032992:c3567314-3566916 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT112941 WP_000554758.1 NZ_CP032992:c3567610-3567317 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT112941 NZ_CP032992:c3567610-3567317 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References