Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1751890..1752392 Replicon chromosome
Accession NZ_CP032992
Organism Escherichia coli strain W5-6

Toxin (Protein)


Gene name yoeB Uniprot ID A0A8E0FJZ4
Locus tag D9V54_RS08475 Protein ID WP_000767833.1
Coordinates 1752138..1752392 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D9V54_RS08470 Protein ID WP_001259255.1
Coordinates 1751890..1752141 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V54_RS08440 1747068..1748135 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
D9V54_RS08445 1748135..1749205 - 1071 WP_000108940.1 histidinol-phosphate transaminase -
D9V54_RS08450 1749208..1750506 - 1299 WP_049290931.1 histidinol dehydrogenase -
D9V54_RS08455 1750512..1751411 - 900 WP_000131756.1 ATP phosphoribosyltransferase -
D9V54_RS08465 1751557..1751607 - 51 WP_001364200.1 his operon leader peptide -
D9V54_RS08470 1751890..1752141 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D9V54_RS08475 1752138..1752392 + 255 WP_000767833.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D9V54_RS08480 1752475..1753299 + 825 WP_000754758.1 SDR family oxidoreductase -
D9V54_RS08485 1753345..1754275 + 931 Protein_1622 LysR family transcriptional regulator -
D9V54_RS08490 1754490..1754552 + 63 WP_010723108.1 membrane protein YoeI -
D9V54_RS08495 1754542..1755900 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D9V54_RS08500 1756079..1757138 + 1060 Protein_1625 transport protein YeeE -
D9V54_RS08505 1757152..1757379 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10173.54 Da        Isoelectric Point: 8.0353

>T112928 WP_000767833.1 NZ_CP032992:1752138-1752392 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPESLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T112928 NZ_CP032992:1752138-1752392 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAATCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT112928 WP_001259255.1 NZ_CP032992:1751890-1752141 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT112928 NZ_CP032992:1751890-1752141 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References