Detailed information of TA system TA112919

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 48578..48831
• Replicon: plasmid p2_W2-5
• Accession: NZ_CP032991
• Organism: Escherichia coli strain W2-5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: D9V55_RS25050
• Protein ID: WP_001312851.1
• Coordinates: 48578..48727 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	48772..48831 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V55_RS25585	43884..43988	-	105	WP_032409716.1	hypothetical protein	-
D9V55_RS25010	44218..44406	+	189	WP_000957857.1	hypothetical protein	-
D9V55_RS25015	44416..45615	+	1200	WP_000948429.1	IS91 family transposase	-
D9V55_RS25590	45779..45937	-	159	WP_047667301.1	membrane protein	-
D9V55_RS25030	46877..47734	-	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
D9V55_RS25035	47727..47801	-	75	WP_001375168.1	RepA leader peptide Tap	-
D9V55_RS25045	48046..48294	-	249	WP_000083837.1	replication regulatory protein RepA	-
D9V55_RS25050	48578..48727	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	48772..48831	+	60	NuclAT_1	-	Antitoxin
-	48772..48831	+	60	NuclAT_1	-	Antitoxin
-	48772..48831	+	60	NuclAT_1	-	Antitoxin
-	48772..48831	+	60	NuclAT_1	-	Antitoxin
D9V55_RS25060	49032..49262	-	231	WP_001736714.1	hypothetical protein	-
D9V55_RS25065	49426..50025	-	600	WP_032083981.1	hypothetical protein	-
D9V55_RS25070	50411..50611	-	201	WP_015059022.1	hypothetical protein	-
D9V55_RS25075	50743..51303	-	561	WP_000139328.1	fertility inhibition protein FinO	-
D9V55_RS25080	51358..52104	-	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..83867	83867
	-	flank	IS/Tn	-	-	44416..45615	1199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T112919 WP_001312851.1 NZ_CP032991:c48727-48578 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T112919 NZ_CP032991:c48727-48578 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT112919 NZ_CP032991:48772-48831 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin