Detailed information of TA system TA112914

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4186..4612
• Replicon: plasmid p2_W2-5
• Accession: NZ_CP032991
• Organism: Escherichia coli strain W2-5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: D9V55_RS24720
• Protein ID: WP_001312861.1
• Coordinates: 4186..4344 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4388..4612 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V55_RS24685	443..824	-	382	Protein_1	relaxosome protein TraM	-
D9V55_RS24690	1238..1748	+	511	Protein_2	transglycosylase SLT domain-containing protein	-
D9V55_RS24695	2045..2865	-	821	Protein_3	DUF932 domain-containing protein	-
D9V55_RS24700	2976..3272	-	297	WP_001272251.1	hypothetical protein	-
D9V55_RS25705	3572..3948	+	377	Protein_5	hypothetical protein	-
D9V55_RS24720	4186..4344	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	4388..4612	-	225	NuclAT_0	-	Antitoxin
-	4388..4612	-	225	NuclAT_0	-	Antitoxin
-	4388..4612	-	225	NuclAT_0	-	Antitoxin
-	4388..4612	-	225	NuclAT_0	-	Antitoxin
D9V55_RS25580	4424..4603	+	180	WP_001309233.1	hypothetical protein	-
D9V55_RS24725	4624..5343	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
D9V55_RS24730	5340..5774	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
D9V55_RS24735	5843..7866	-	2024	Protein_10	ParB/RepB/Spo0J family partition protein	-
D9V55_RS24740	7927..8160	-	234	WP_000006003.1	DUF905 family protein	-
D9V55_RS24745	8218..8745	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-55	-	1..83867	83867

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T112914 WP_001312861.1 NZ_CP032991:c4344-4186 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T112914 NZ_CP032991:c4344-4186 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT112914 NZ_CP032991:c4612-4388 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin