Detailed information of TA system TA112910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4709535..4710137
• Replicon: chromosome
• Accession: NZ_CP032989
• Organism: Escherichia coli strain W2-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: D9V55_RS23245
• Protein ID: WP_000897305.1
• Coordinates: 4709826..4710137 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D9V55_RS23240
• Protein ID: WP_000356397.1
• Coordinates: 4709535..4709825 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V55_RS23210	4705461..4706363	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
D9V55_RS23215	4706360..4706995	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
D9V55_RS23220	4706992..4707921	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
D9V55_RS23225	4708251..4708493	-	243	WP_001086388.1	hypothetical protein	-
D9V55_RS23230	4708712..4708930	-	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
D9V55_RS23240	4709535..4709825	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
D9V55_RS23245	4709826..4710137	-	312	WP_000897305.1	hypothetical protein	Toxin
D9V55_RS23250	4710366..4711274	+	909	WP_001162704.1	alpha/beta hydrolase	-
D9V55_RS23255	4711338..4712279	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
D9V55_RS23260	4712324..4712761	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D9V55_RS23265	4712758..4713630	-	873	WP_000920762.1	virulence factor BrkB family protein	-
D9V55_RS23270	4713624..4714223	-	600	WP_001295269.1	glucose-1-phosphatase	-
D9V55_RS23275	4714322..4715107	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T112910 WP_000897305.1 NZ_CP032989:c4710137-4709826 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T112910 NZ_CP032989:c4710137-4709826 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT112910 WP_000356397.1 NZ_CP032989:c4709825-4709535 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT112910 NZ_CP032989:c4709825-4709535 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7