Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1947590..1948092 Replicon chromosome
Accession NZ_CP032989
Organism Escherichia coli strain W2-5

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D9V55_RS09710 Protein ID WP_000767829.1
Coordinates 1947838..1948092 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D9V55_RS09705 Protein ID WP_001259255.1
Coordinates 1947590..1947841 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9V55_RS09675 1942768..1943835 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
D9V55_RS09680 1943835..1944905 - 1071 WP_032203184.1 histidinol-phosphate transaminase -
D9V55_RS09685 1944902..1946206 - 1305 WP_001774536.1 histidinol dehydrogenase -
D9V55_RS09690 1946212..1947111 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
D9V55_RS09700 1947257..1947307 - 51 WP_001364200.1 his operon leader peptide -
D9V55_RS09705 1947590..1947841 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D9V55_RS09710 1947838..1948092 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D9V55_RS09715 1948175..1948999 + 825 WP_000754758.1 SDR family oxidoreductase -
D9V55_RS09720 1949045..1949974 + 930 WP_000803351.1 LysR family transcriptional regulator -
D9V55_RS09725 1950189..1950251 + 63 WP_010723108.1 membrane protein YoeI -
D9V55_RS09730 1950241..1951599 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D9V55_RS09735 1951778..1952836 + 1059 WP_000492315.1 transport protein YeeE -
D9V55_RS09740 1952850..1953077 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T112894 WP_000767829.1 NZ_CP032989:1947838-1948092 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T112894 NZ_CP032989:1947838-1948092 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT112894 WP_001259255.1 NZ_CP032989:1947590-1947841 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT112894 NZ_CP032989:1947590-1947841 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References