Detailed information of TA system TA112881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3623091..3623785
• Replicon: chromosome
• Accession: NZ_CP032986
• Organism: Escherichia coli strain BE2-5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: D9V56_RS17940
• Protein ID: WP_001263493.1
• Coordinates: 3623091..3623489 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: D9V56_RS17945
• Protein ID: WP_000554757.1
• Coordinates: 3623492..3623785 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V56_RS17910	3618091..3619335	-	1245	WP_000189541.1	esterase FrsA	-
-	3618751..3618831	-	81	NuclAT_13	-	-
-	3618751..3618831	-	81	NuclAT_13	-	-
-	3618751..3618831	-	81	NuclAT_13	-	-
-	3618751..3618831	-	81	NuclAT_13	-	-
D9V56_RS17915	3619427..3619885	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D9V56_RS17920	3620146..3621603	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
D9V56_RS17925	3621660..3622181	-	522	Protein_3397	peptide chain release factor H	-
D9V56_RS17930	3622180..3622383	-	204	Protein_3398	RNA ligase RtcB family protein	-
D9V56_RS17935	3622629..3623081	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
D9V56_RS17940	3623091..3623489	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D9V56_RS17945	3623492..3623785	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D9V56_RS17950	3623837..3624892	-	1056	WP_085452615.1	DNA polymerase IV	-
D9V56_RS17955	3624963..3625748	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
D9V56_RS17960	3625720..3627432	+	1713	Protein_3404	flagellar biosynthesis protein FlhA	-
D9V56_RS17965	3627648..3628145	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T112881 WP_001263493.1 NZ_CP032986:c3623489-3623091 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T112881 NZ_CP032986:c3623489-3623091 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT112881 WP_000554757.1 NZ_CP032986:c3623785-3623492 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT112881 NZ_CP032986:c3623785-3623492 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6