Detailed information of TA system TA112864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 640389..641188
• Replicon: chromosome
• Accession: NZ_CP032986
• Organism: Escherichia coli strain BE2-5

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: D9V56_RS03200
• Protein ID: WP_000347273.1
• Coordinates: 640389..640853 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0TI39
• Locus tag: D9V56_RS03205
• Protein ID: WP_021547164.1
• Coordinates: 640853..641188 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9V56_RS03170	635390..635824	-	435	WP_000948814.1	PTS sugar transporter subunit IIA	-
D9V56_RS03175	635842..636720	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
D9V56_RS03180	636710..637489	-	780	WP_077492473.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
D9V56_RS03185	637500..637973	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
D9V56_RS03190	637996..639276	-	1281	WP_042096485.1	tagatose-bisphosphate aldolase subunit KbaZ	-
D9V56_RS03195	639525..640334	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
D9V56_RS03200	640389..640853	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
D9V56_RS03205	640853..641188	-	336	WP_021547164.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
D9V56_RS03210	641337..642908	-	1572	WP_001753084.1	galactarate dehydratase	-
D9V56_RS03215	643283..644617	+	1335	WP_077492475.1	galactarate/glucarate/glycerate transporter GarP	-
D9V56_RS03220	644633..645403	+	771	WP_001723934.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T112864 WP_000347273.1 NZ_CP032986:c640853-640389 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T112864 NZ_CP032986:c640853-640389 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGGCATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12309.86 Da
• Isoelectric Point: 4.5392

>AT112864 WP_021547164.1 NZ_CP032986:c641188-640853 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT112864 NZ_CP032986:c641188-640853 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: V0TI39