Detailed information of TA system TA112834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 459222..459810
• Replicon: chromosome
• Accession: NZ_CP032911
• Organism: Helicobacter pylori strain 19-A-EK3

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: K2LF18
• Locus tag: D4H43_RS02330
• Protein ID: WP_000921926.1
• Coordinates: 459222..459503 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: D4H43_RS02335
• Protein ID: WP_139543876.1
• Coordinates: 459496..459810 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4H43_RS02295	454725..455282	+	558	WP_164500424.1	hypothetical protein	-
D4H43_RS02300	455333..455620	+	288	WP_053574735.1	hypothetical protein	-
D4H43_RS02305	455890..456013	+	124	Protein_430	DNA adenine methylase	-
D4H43_RS02310	456227..457051	+	825	WP_097568619.1	mechanosensitive ion channel family protein	-
D4H43_RS08360	457339..457523	+	185	Protein_432	hypothetical protein	-
D4H43_RS02320	457704..457853	-	150	WP_127985952.1	type II restriction endonuclease	-
D4H43_RS02325	457893..458999	-	1107	WP_128008304.1	DUF1887 family protein	-
D4H43_RS02330	459222..459503	-	282	WP_000921926.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
D4H43_RS02335	459496..459810	-	315	WP_139543876.1	hypothetical protein	Antitoxin
D4H43_RS02340	460092..462003	+	1912	Protein_437	relaxase/mobilization nuclease domain-containing protein	-
D4H43_RS02345	462027..463211	-	1185	WP_139543877.1	hypothetical protein	-
D4H43_RS02350	463255..463539	-	285	WP_000394651.1	hypothetical protein	-
D4H43_RS02355	463622..464278	-	657	WP_139543878.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	457929..485689	27760

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10932.89 Da
• Isoelectric Point: 9.8257

>T112834 WP_000921926.1 NZ_CP032911:c459503-459222 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRIKESDVTSIVDCLKEQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T112834 NZ_CP032911:c459503-459222 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTAAAGAAAGCGA
TGTTACAAGCATTGTTGATTGCCTCAAAGAGCAAAAGCCACTCCAACAAAAATATTGCGACCATGCCTTGAGTGGCAATC
TTAAAGGGCTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12270.77 Da
• Isoelectric Point: 5.7750

>AT112834 WP_139543876.1 NZ_CP032911:c459810-459496 [Helicobacter pylori]
MSNVITDYSQYNEKQLHNFLNSIEKQLLKAERDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
CDVSFGDFDDFLQIAKQERERRNA

Nucleotide

• Length: 315 bp

>AT112834 NZ_CP032911:c459810-459496 [Helicobacter pylori]
ATGTCAAATGTTATTACAGATTACTCGCAATACAATGAAAAGCAACTGCACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAAGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAGG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGACCCAGAA
TGCGATGTTTCGTTTGGAGACTTTGATGATTTTTTACAAATAGCAAAACAAGAAAGAGAAAGGCGTAATGCTTAA

Structures

Toxin

• AlphaFold DB: K2LF18