Detailed information of TA system TA112827

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ(toxin)
• Location: 1111792..1112380
• Replicon: chromosome
• Accession: NZ_CP032908
• Organism: Helicobacter pylori strain 23-A-EK1

Toxin (Protein)

• Gene name: TfiT
• Uniprot ID: A0A496ESF5
• Locus tag: D4I31_RS05385
• Protein ID: WP_033756152.1
• Coordinates: 1111792..1112073 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: TfiA
• Uniprot ID: -
• Locus tag: D4I31_RS05390
• Protein ID: WP_139534945.1
• Coordinates: 1112066..1112380 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D4I31_RS05350	1106996..1107259	+	264	WP_139534942.1	hypothetical protein	-
D4I31_RS05355	1107256..1107555	+	300	WP_000660136.1	hypothetical protein	-
D4I31_RS05360	1107555..1108442	+	888	WP_025366525.1	Flp pilus assembly complex ATPase component TadA	-
D4I31_RS05365	1108492..1108947	+	456	WP_139534943.1	mobilization protein	-
D4I31_RS05370	1108944..1109234	+	291	WP_000159810.1	hypothetical protein	-
D4I31_RS05375	1109235..1109792	+	558	WP_139534944.1	hypothetical protein	-
D4I31_RS05380	1109793..1111520	+	1728	WP_201738121.1	type IV secretory system conjugative DNA transfer family protein	-
D4I31_RS05385	1111792..1112073	-	282	WP_033756152.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
D4I31_RS05390	1112066..1112380	-	315	WP_139534945.1	hypothetical protein	Antitoxin
D4I31_RS05395	1112719..1114629	+	1911	WP_139534946.1	relaxase/mobilization nuclease domain-containing protein	-
D4I31_RS05400	1114654..1115832	-	1179	WP_139534947.1	hypothetical protein	-
D4I31_RS05405	1115876..1116160	-	285	WP_000394655.1	hypothetical protein	-
D4I31_RS05410	1116242..1116898	-	657	WP_139534948.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	pseB / lpxB / flgE_1	1061454..1134860	73406

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10874.86 Da
• Isoelectric Point: 10.0264

>T112827 WP_033756152.1 NZ_CP032908:c1112073-1111792 [Helicobacter pylori]
MLKVRTKKDFLKDFNKHILSGRITESDVASIVDCLKKQKPLQQKYCDHALSGNLKGLRECHVKPNLLLIYEIKKQENELV
LLRLDTHSELFKK

Nucleotide

• Length: 282 bp

>T112827 NZ_CP032908:c1112073-1111792 [Helicobacter pylori]
ATGCTTAAAGTCAGAACTAAGAAAGATTTTCTTAAAGACTTTAATAAGCATATCTTATCCGGGCGTATTACAGAAAGCGA
TGTTGCAAGCATTGTTGATTGCCTAAAAAAACAAAAACCACTCCAACAAAAATATTGCGACCATGCCTTGAGCGGTAATC
TTAAAGGGTTGAGAGAGTGTCATGTCAAGCCAAACTTGTTATTGATTTATGAAATCAAAAAACAAGAAAACGAACTTGTT
TTATTGCGTCTAGACACTCATAGCGAGCTATTTAAAAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12251.70 Da
• Isoelectric Point: 5.2250

>AT112827 WP_139534945.1 NZ_CP032908:c1112380-1112066 [Helicobacter pylori]
MSNIITDYSQYNEKQLYNFLNSIEKQLLKAEGDKNKAIKKIQECELQERMIRQVLAQKHSQEKEPTQSLLNTIASKDDPE
YDVSFGDFNDFLQIAKQERERHNA

Nucleotide

• Length: 315 bp

>AT112827 NZ_CP032908:c1112380-1112066 [Helicobacter pylori]
ATGTCAAATATTATTACAGATTACTCGCAATACAATGAAAAGCAACTGTACAATTTCTTAAACTCCATTGAAAAACAATT
GCTTAAGGCAGAAGGGGATAAAAATAAGGCGATAAAAAAAATCCAAGAGTGTGAATTGCAAGAACGAATGATCAGACAGG
TTTTAGCCCAAAAACATTCTCAAGAAAAAGAACCCACACAAAGTTTGTTAAATACGATCGCCTCAAAAGATGATCCAGAA
TACGATGTTTCGTTTGGAGACTTCAATGATTTTTTGCAAATAGCGAAACAAGAAAGAGAAAGGCATAATGCTTAA

Structures

Toxin

• AlphaFold DB: A0A496ESF5