Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1890246..1890748 Replicon chromosome
Accession NZ_CP032892
Organism Escherichia coli strain SCEC020022

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7V31_RS12260 Protein ID WP_000767829.1
Coordinates 1890494..1890748 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7V31_RS12255 Protein ID WP_001259255.1
Coordinates 1890246..1890497 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7V31_RS12225 1885424..1886491 - 1068 WP_063078857.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C7V31_RS12230 1886491..1887561 - 1071 WP_063078858.1 histidinol-phosphate transaminase -
C7V31_RS12235 1887558..1888862 - 1305 WP_001572810.1 histidinol dehydrogenase -
C7V31_RS12240 1888868..1889767 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7V31_RS12250 1889913..1889963 - 51 WP_001364200.1 his operon leader peptide -
C7V31_RS12255 1890246..1890497 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7V31_RS12260 1890494..1890748 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7V31_RS12265 1890831..1891655 + 825 WP_000754737.1 SDR family oxidoreductase -
C7V31_RS12270 1891701..1892630 + 930 WP_063078859.1 LysR family transcriptional regulator -
C7V31_RS12275 1892845..1892907 + 63 WP_010723108.1 membrane protein YoeI -
C7V31_RS12280 1892897..1894255 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7V31_RS12285 1894434..1895492 + 1059 WP_000492340.1 transport protein YeeE -
C7V31_RS12290 1895506..1895733 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T112774 WP_000767829.1 NZ_CP032892:1890494-1890748 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T112774 NZ_CP032892:1890494-1890748 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT112774 WP_001259255.1 NZ_CP032892:1890246-1890497 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT112774 NZ_CP032892:1890246-1890497 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References