Detailed information of TA system TA112763

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 41920..42346
• Replicon: plasmid pVir_020022
• Accession: NZ_CP032891
• Organism: Escherichia coli strain SCEC020022

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C7V31_RS02755
• Protein ID: WP_001312861.1
• Coordinates: 41920..42078 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	42122..42346 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V31_RS02695	37091..37636	-	546	WP_000059851.1	type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB	-
C7V31_RS02700	37623..37907	-	285	WP_000624109.1	type-F conjugative transfer system pilin chaperone TraQ	-
C7V31_RS02705	37988..38302	+	315	WP_001324103.1	hypothetical protein	-
C7V31_RS02710	38304..38651	-	348	WP_001287910.1	conjugal transfer protein TrbA	-
C7V31_RS02715	38667..39410	-	744	WP_001030393.1	type-F conjugative transfer system pilin assembly protein TraF	-
C7V31_RS27570	39403..39546	-	144	Protein_42	conjugal transfer protein TrbE	-
C7V31_RS02730	39593..40297	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C7V31_RS02735	40361..40594	-	234	Protein_44	DUF932 domain-containing protein	-
C7V31_RS02740	40712..40999	-	288	WP_000107535.1	hypothetical protein	-
C7V31_RS02755	41920..42078	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	42122..42346	-	225	NuclAT_0	-	Antitoxin
-	42122..42346	-	225	NuclAT_0	-	Antitoxin
-	42122..42346	-	225	NuclAT_0	-	Antitoxin
-	42122..42346	-	225	NuclAT_0	-	Antitoxin
C7V31_RS27220	42158..42346	+	189	WP_001299721.1	hypothetical protein	-
C7V31_RS02760	42358..43077	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
C7V31_RS02765	43074..43508	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
C7V31_RS02770	43563..43760	-	198	Protein_50	hypothetical protein	-
C7V31_RS02775	43788..44021	-	234	WP_000005987.1	DUF905 family protein	-
C7V31_RS02780	44089..44628	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
C7V31_RS27575	44654..44860	-	207	WP_000275856.1	hypothetical protein	-
C7V31_RS02805	45999..46970	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA	1..67351	67351
	-	flank	IS/Tn	-	-	39593..40297	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T112763 WP_001312861.1 NZ_CP032891:c42078-41920 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T112763 NZ_CP032891:c42078-41920 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT112763 NZ_CP032891:c42346-42122 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin