Detailed information of TA system TA112758

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 16479..16733
• Replicon: plasmid pVir_020022
• Accession: NZ_CP032891
• Organism: Escherichia coli strain SCEC020022

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: C7V31_RS02600
• Protein ID: WP_001351576.1
• Coordinates: 16479..16685 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	16672..16733 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V31_RS02560	11723..12420	+	698	Protein_12	IS1 family transposase	-
C7V31_RS02565	12498..13887	-	1390	Protein_13	ISNCY family transposase	-
C7V31_RS02570	13975..14679	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
C7V31_RS02575	14733..15164	-	432	Protein_15	incFII family plasmid replication initiator RepA	-
C7V31_RS02580	15157..15639	-	483	WP_001273588.1	hypothetical protein	-
C7V31_RS02585	15632..15706	-	75	WP_001442103.1	RepA leader peptide Tap	-
C7V31_RS02595	15938..16195	-	258	WP_000083845.1	replication regulatory protein RepA	-
C7V31_RS02600	16479..16685	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	16672..16733	+	62	NuclAT_1	-	Antitoxin
-	16672..16733	+	62	NuclAT_1	-	Antitoxin
-	16672..16733	+	62	NuclAT_1	-	Antitoxin
-	16672..16733	+	62	NuclAT_1	-	Antitoxin
C7V31_RS02610	17095..18636	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
C7V31_RS02615	18651..19397	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
C7V31_RS27565	19577..19651	-	75	Protein_22	endonuclease	-
C7V31_RS02620	19897..20109	-	213	WP_001299730.1	ANR family transcriptional regulator	-
C7V31_RS02625	20245..20805	-	561	WP_000139315.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA	1..67351	67351
	-	inside	IScluster/Tn	-	-	7767..19397	11630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T112758 WP_001351576.1 NZ_CP032891:c16685-16479 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T112758 NZ_CP032891:c16685-16479 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT112758 NZ_CP032891:16672-16733 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin