Detailed information of TA system TA112753

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63174..63438
• Replicon: plasmid pCTXM14_020022
• Accession: NZ_CP032888
• Organism: Escherichia coli strain SCEC020022

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: C7V31_RS01260
• Protein ID: WP_001303307.1
• Coordinates: 63286..63438 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	63174..63236 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V31_RS01245	59276..60346	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
C7V31_RS01250	60365..61573	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	61753..61813	-	61	NuclAT_1	-	-
-	61753..61813	-	61	NuclAT_1	-	-
-	61753..61813	-	61	NuclAT_1	-	-
-	61753..61813	-	61	NuclAT_1	-	-
C7V31_RS01255	61880..62971	-	1092	WP_000426061.1	hypothetical protein	-
-	63174..63236	-	63	NuclAT_0	-	Antitoxin
-	63174..63236	-	63	NuclAT_0	-	Antitoxin
-	63174..63236	-	63	NuclAT_0	-	Antitoxin
-	63174..63236	-	63	NuclAT_0	-	Antitoxin
C7V31_RS01260	63286..63438	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
C7V31_RS01265	63510..63761	-	252	WP_001291968.1	hypothetical protein	-
-	64148..64199	-	52	NuclAT_2	-	-
-	64148..64199	-	52	NuclAT_2	-	-
-	64148..64199	-	52	NuclAT_2	-	-
-	64148..64199	-	52	NuclAT_2	-	-
C7V31_RS27195	64685..64861	-	177	WP_001054900.1	hypothetical protein	-
C7V31_RS01280	65070..65279	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
C7V31_RS01285	65377..65991	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
C7V31_RS01290	66067..68235	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / blaCTX-M-14b	-	1..109553	109553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T112753 WP_001303307.1 NZ_CP032888:63286-63438 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T112753 NZ_CP032888:63286-63438 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT112753 NZ_CP032888:c63236-63174 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin