Detailed information of TA system TA112746

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3981971..3982665
• Replicon: chromosome
• Accession: NZ_CP032879
• Organism: Escherichia coli strain WCHEC000837

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: D9N32_RS21815
• Protein ID: WP_001521903.1
• Coordinates: 3982267..3982665 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: D9N32_RS21810
• Protein ID: WP_000554758.1
• Coordinates: 3981971..3982264 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N32_RS21785	3977086..3977835	+	750	WP_016233951.1	C40 family peptidase	-
D9N32_RS21790	3977892..3978245	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
D9N32_RS21795	3978324..3980036	-	1713	Protein_3710	flagellar biosynthesis protein FlhA	-
D9N32_RS21800	3980008..3980793	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
D9N32_RS21805	3980864..3981919	+	1056	WP_001226177.1	DNA polymerase IV	-
D9N32_RS21810	3981971..3982264	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
D9N32_RS21815	3982267..3982665	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
D9N32_RS21820	3982675..3983127	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
D9N32_RS21825	3983372..3983578	+	207	Protein_3716	RtcB family protein	-
D9N32_RS21830	3983574..3983926	+	353	Protein_3717	peptide chain release factor H	-
D9N32_RS21835	3983983..3985440	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
D9N32_RS21840	3985701..3986159	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
D9N32_RS21845	3986251..3987495	+	1245	WP_000189541.1	esterase FrsA	-
-	3986755..3986835	+	81	NuclAT_10	-	-
-	3986755..3986835	+	81	NuclAT_10	-	-
-	3986755..3986835	+	81	NuclAT_10	-	-
-	3986755..3986835	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T112746 WP_001521903.1 NZ_CP032879:3982267-3982665 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T112746 NZ_CP032879:3982267-3982665 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT112746 WP_000554758.1 NZ_CP032879:3981971-3982264 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT112746 NZ_CP032879:3981971-3982264 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3