Detailed information of TA system TA112739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3025397..3025999
• Replicon: chromosome
• Accession: NZ_CP032879
• Organism: Escherichia coli strain WCHEC000837

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: D9N32_RS17065
• Protein ID: WP_000897305.1
• Coordinates: 3025397..3025708 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D9N32_RS17070
• Protein ID: WP_000356395.1
• Coordinates: 3025709..3025999 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N32_RS17040	3020438..3020875	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
D9N32_RS17045	3020920..3021861	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
D9N32_RS17050	3022277..3024091	+	1815	WP_020233236.1	hypothetical protein	-
D9N32_RS17060	3024260..3025168	-	909	WP_001306651.1	alpha/beta hydrolase	-
D9N32_RS17065	3025397..3025708	+	312	WP_000897305.1	hypothetical protein	Toxin
D9N32_RS17070	3025709..3025999	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
D9N32_RS17080	3026358..3026636	+	279	WP_001306650.1	hypothetical protein	-
D9N32_RS17090	3027055..3027273	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
D9N32_RS17095	3027492..3027734	+	243	WP_021523315.1	hypothetical protein	-
D9N32_RS17100	3027916..3028845	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
D9N32_RS17105	3028842..3029477	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
D9N32_RS17110	3029474..3030376	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T112739 WP_000897305.1 NZ_CP032879:3025397-3025708 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T112739 NZ_CP032879:3025397-3025708 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT112739 WP_000356395.1 NZ_CP032879:3025709-3025999 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT112739 NZ_CP032879:3025709-3025999 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7