Detailed information of TA system TA112738

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2190020..2190819
• Replicon: chromosome
• Accession: NZ_CP032879
• Organism: Escherichia coli strain WCHEC000837

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: D3GWU5
• Locus tag: D9N32_RS12915
• Protein ID: WP_000347272.1
• Coordinates: 2190355..2190819 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: D9N32_RS12910
• Protein ID: WP_001307405.1
• Coordinates: 2190020..2190355 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N32_RS12895	2185805..2186575	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
D9N32_RS12900	2186591..2187925	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
D9N32_RS12905	2188300..2189871	+	1572	WP_001273738.1	galactarate dehydratase	-
D9N32_RS12910	2190020..2190355	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
D9N32_RS12915	2190355..2190819	+	465	WP_000347272.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
D9N32_RS12920	2190874..2191683	-	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
D9N32_RS12925	2191932..2193212	+	1281	WP_001521382.1	tagatose-bisphosphate aldolase subunit KbaZ	-
D9N32_RS12930	2193235..2193708	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
D9N32_RS12935	2193719..2194498	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
D9N32_RS12940	2194488..2195366	+	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
D9N32_RS12945	2195384..2195818	+	435	WP_000948824.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2180495..2190819	10324

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17832.26 Da
• Isoelectric Point: 9.6924

>T112738 WP_000347272.1 NZ_CP032879:2190355-2190819 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Nucleotide

• Length: 465 bp

>T112738 NZ_CP032879:2190355-2190819 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT112738 WP_001307405.1 NZ_CP032879:2190020-2190355 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT112738 NZ_CP032879:2190020-2190355 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829KUD2

Antitoxin

• AlphaFold DB: A0A0E0XVC7