Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 881596..882098 Replicon chromosome
Accession NZ_CP032879
Organism Escherichia coli strain WCHEC000837

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag D9N32_RS06460 Protein ID WP_000767829.1
Coordinates 881596..881850 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag D9N32_RS06465 Protein ID WP_001259255.1
Coordinates 881847..882098 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9N32_RS06430 876611..876838 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
D9N32_RS06435 876852..877910 - 1059 WP_000492324.1 transport protein YeeE -
D9N32_RS06440 878089..879447 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
D9N32_RS06445 879437..879499 - 63 WP_010723108.1 membrane protein YoeI -
D9N32_RS06450 879714..880643 - 930 WP_023154044.1 LysR family transcriptional regulator -
D9N32_RS06455 880689..881513 - 825 WP_000754758.1 SDR family oxidoreductase -
D9N32_RS06460 881596..881850 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
D9N32_RS06465 881847..882098 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
D9N32_RS06470 882381..882431 + 51 WP_001364200.1 his operon leader peptide -
D9N32_RS06480 882577..883476 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
D9N32_RS06485 883482..884786 + 1305 WP_021523144.1 histidinol dehydrogenase -
D9N32_RS06490 884783..885853 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
D9N32_RS06495 885853..886920 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T112732 WP_000767829.1 NZ_CP032879:c881850-881596 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T112732 NZ_CP032879:c881850-881596 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT112732 WP_001259255.1 NZ_CP032879:c882098-881847 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT112732 NZ_CP032879:c882098-881847 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References