Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 46925..47179 Replicon plasmid pCTXM15_000837
Accession NZ_CP032877
Organism Escherichia coli strain WCHEC000837

Toxin (Protein)


Gene name srnB Uniprot ID -
Locus tag D9N32_RS00945 Protein ID WP_015387399.1
Coordinates 46973..47179 (+) Length 69 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 46925..46986 (-)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D9N32_RS00900 42577..42789 + 213 WP_005012601.1 hypothetical protein -
D9N32_RS27740 43090..43179 - 90 Protein_48 IS1 family transposase -
D9N32_RS00915 43234..43911 + 678 WP_001339397.1 IS66 family insertion sequence hypothetical protein -
D9N32_RS00920 43911..44258 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
D9N32_RS00925 44278..45849 + 1572 WP_023149734.1 IS66 family transposase -
D9N32_RS00930 45887..46503 - 617 Protein_52 IS1 family transposase -
D9N32_RS00935 46604..46786 + 183 WP_000968309.1 hypothetical protein -
- 46925..46986 - 62 NuclAT_2 - Antitoxin
- 46925..46986 - 62 NuclAT_2 - Antitoxin
- 46925..46986 - 62 NuclAT_2 - Antitoxin
- 46925..46986 - 62 NuclAT_2 - Antitoxin
D9N32_RS00945 46973..47179 + 207 WP_015387399.1 type I toxin-antitoxin system Hok family toxin Toxin
D9N32_RS00950 47463..47720 + 258 WP_000083833.1 replication regulatory protein RepA -
D9N32_RS00960 47956..48030 + 75 WP_032336874.1 RepA leader peptide Tap -
D9N32_RS00965 48023..48469 + 447 Protein_57 incFII family plasmid replication initiator RepA -
D9N32_RS00970 48415..48897 - 483 Protein_58 VENN motif pre-toxin domain-containing protein -
D9N32_RS00975 49790..51010 + 1221 WP_000410951.1 arginine deiminase -
D9N32_RS00980 51021..51932 + 912 WP_000440183.1 carbamate kinase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / aadA5 / qacE / sul1 / mph(A) / tet(B) - 1..150853 150853


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(23-64)


Sequences


Toxin        


Download         Length: 69 a.a.        Molecular weight: 7720.23 Da        Isoelectric Point: 9.3127

>T112716 WP_015387399.1 NZ_CP032877:46973-47179 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 207 bp

>T112716 NZ_CP032877:46973-47179 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 62 bp

>AT112716 NZ_CP032877:c46986-46925 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Download structure file

References