Detailed information of TA system TA112712

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4527..4760
• Replicon: plasmid pCTXM15_000837
• Accession: NZ_CP032877
• Organism: Escherichia coli strain WCHEC000837

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: D9N32_RS00690
• Protein ID: WP_001312861.1
• Coordinates: 4602..4760 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	4527..4558 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N32_RS00660	606..1145	+	540	WP_000290840.1	single-stranded DNA-binding protein	-
D9N32_RS00665	1213..1446	+	234	WP_000005990.1	DUF905 family protein	-
D9N32_RS00670	1474..1671	+	198	Protein_2	hypothetical protein	-
D9N32_RS00675	1726..2160	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
D9N32_RS00680	2157..2876	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
D9N32_RS27405	2888..3076	-	189	WP_001299721.1	hypothetical protein	-
-	2888..3085	+	198	NuclAT_0	-	-
-	2888..3085	+	198	NuclAT_0	-	-
-	2888..3085	+	198	NuclAT_0	-	-
-	2888..3085	+	198	NuclAT_0	-	-
D9N32_RS00685	3133..4502	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	4527..4558	+	32	NuclAT_1	-	Antitoxin
-	4527..4558	+	32	NuclAT_1	-	Antitoxin
-	4527..4558	+	32	NuclAT_1	-	Antitoxin
-	4527..4558	+	32	NuclAT_1	-	Antitoxin
D9N32_RS00690	4602..4760	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
D9N32_RS00695	5155..6822	-	1668	WP_012372796.1	group II intron reverse transcriptase/maturase	-
D9N32_RS00705	8024..8311	+	288	WP_000107535.1	hypothetical protein	-
D9N32_RS00710	8429..9250	+	822	WP_001234426.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / aadA5 / qacE / sul1 / mph(A) / tet(B)	-	1..150853	150853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T112712 WP_001312861.1 NZ_CP032877:4602-4760 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T112712 NZ_CP032877:4602-4760 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT112712 NZ_CP032877:4527-4558 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin