Detailed information of TA system TA112710

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 34829..35400
• Replicon: plasmid p1_000837
• Accession: NZ_CP032875
• Organism: Escherichia coli strain WCHEC000837

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: D9N32_RS00215
• Protein ID: WP_032323275.1
• Coordinates: 35119..35400 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D9N32_RS00210
• Protein ID: WP_032323276.1
• Coordinates: 34829..35110 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N32_RS00175	30515..31306	-	792	WP_063074813.1	hypothetical protein	-
D9N32_RS00180	31469..31771	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D9N32_RS00185	31842..32180	+	339	WP_000806445.1	HigA family addiction module antidote protein	-
D9N32_RS00190	32237..32437	+	201	WP_001022420.1	hypothetical protein	-
D9N32_RS00195	32459..33187	+	729	WP_080077649.1	hypothetical protein	-
D9N32_RS00200	33221..34414	-	1194	WP_063074560.1	hypothetical protein	-
D9N32_RS00205	34407..34736	-	330	WP_000542383.1	hypothetical protein	-
D9N32_RS00210	34829..35110	-	282	WP_032323276.1	HigA family addiction module antidote protein	Antitoxin
D9N32_RS00215	35119..35400	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9N32_RS00220	35737..36390	-	654	WP_000410954.1	hypothetical protein	-
D9N32_RS00225	36674..37282	+	609	WP_032323273.1	hypothetical protein	-
D9N32_RS00230	37468..37695	+	228	WP_001396850.1	hypothetical protein	-
D9N32_RS00235	37692..38003	-	312	WP_000717724.1	hypothetical protein	-
D9N32_RS00240	38063..38989	-	927	WP_072275558.1	Rha family transcriptional regulator	-
D9N32_RS00245	38989..39204	-	216	WP_022630900.1	host cell division inhibitor Icd-like protein	-
D9N32_RS00250	39223..39342	-	120	WP_071526433.1	ash family protein	-
D9N32_RS27390	39516..39683	-	168	WP_162983138.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92538	92538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-86)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10606.14 Da
• Isoelectric Point: 10.2589

>T112710 WP_032323275.1 NZ_CP032875:c35400-35119 [Escherichia coli]
MIKSFRHKGLERFFKTGSTAGIQAKHAVKLRIQLTALNTAKRPDDMSAPGWELHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T112710 NZ_CP032875:c35400-35119 [Escherichia coli]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACGCTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTGACTGCGCTTAATACAGCTAAACGCCCGGACGATATGTCTGCGCCTGGATGGGAATTGC
ACCCTCTAAAGGGGGCGGACCTTAAAGGTCACTGGGCAATATCGGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGAGAAGACGCAATTCTAGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10160.63 Da
• Isoelectric Point: 7.3179

>AT112710 WP_032323276.1 NZ_CP032875:c35110-34829 [Escherichia coli]
MSRMHNPAHPGAVLREYLGDMSVTDAAAALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TTRPNIRPLFFHA

Nucleotide

• Length: 282 bp

>AT112710 NZ_CP032875:c35110-34829 [Escherichia coli]
ATGAGTCGTATGCATAATCCCGCGCATCCAGGCGCGGTTCTTCGTGAATATTTGGGAGACATGTCAGTCACAGACGCCGC
TGCGGCATTAGGCATTACTCGCACGGCGCTTTCCCGAATTCTGAATGAAAAAGCAGGTATATCTGCTGATATGGCACTTC
GTCTGGAGTCCGCTCTTGGAACCAGTGCCGAAATGTGGACCGGTATGCAATCCCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCACTCGCCCAAACATCCGTCCATTATTTTTCCACGCATAA