Detailed information of TA system TA112709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 31469..32180
• Replicon: plasmid p1_000837
• Accession: NZ_CP032875
• Organism: Escherichia coli strain WCHEC000837

Toxin (Protein)

• Gene name: higB
• Uniprot ID: I2UJZ0
• Locus tag: D9N32_RS00180
• Protein ID: WP_000162415.1
• Coordinates: 31469..31771 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: D9N32_RS00185
• Protein ID: WP_000806445.1
• Coordinates: 31842..32180 (+)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N32_RS00150	26488..27168	-	681	WP_000812238.1	hypothetical protein	-
D9N32_RS00155	27152..28057	-	906	WP_087503502.1	recombination-associated protein RdgC	-
D9N32_RS00160	28117..28770	-	654	WP_087503501.1	baseplate protein	-
D9N32_RS00165	28767..29747	-	981	WP_069936439.1	tail length tape measure protein	-
D9N32_RS00170	29751..30518	-	768	WP_000203293.1	hypothetical protein	-
D9N32_RS00175	30515..31306	-	792	WP_063074813.1	hypothetical protein	-
D9N32_RS00180	31469..31771	+	303	WP_000162415.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9N32_RS00185	31842..32180	+	339	WP_000806445.1	HigA family addiction module antidote protein	Antitoxin
D9N32_RS00190	32237..32437	+	201	WP_001022420.1	hypothetical protein	-
D9N32_RS00195	32459..33187	+	729	WP_080077649.1	hypothetical protein	-
D9N32_RS00200	33221..34414	-	1194	WP_063074560.1	hypothetical protein	-
D9N32_RS00205	34407..34736	-	330	WP_000542383.1	hypothetical protein	-
D9N32_RS00210	34829..35110	-	282	WP_032323276.1	HigA family addiction module antidote protein	-
D9N32_RS00215	35119..35400	-	282	WP_032323275.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D9N32_RS00220	35737..36390	-	654	WP_000410954.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..92538	92538

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (6-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11818.56 Da
• Isoelectric Point: 9.8739

>T112709 WP_000162415.1 NZ_CP032875:31469-31771 [Escherichia coli]
MTKKINIKDFRDAWLDDFFEFSTPHRKIPPDIHMTLSRKLDIINAATTCKDLRSPPGNRYEELSGKLNGYSSVRVNKQYR
LIFKWVNGKAEDLYLDPHKY

Nucleotide

• Length: 303 bp

>T112709 NZ_CP032875:31469-31771 [Escherichia coli]
ATGACCAAAAAAATTAACATAAAGGATTTCAGGGATGCGTGGCTTGATGATTTTTTTGAATTTTCAACACCACATAGAAA
GATACCTCCTGATATTCATATGACATTGTCACGGAAGTTGGACATTATCAATGCCGCAACTACCTGTAAGGATTTAAGAT
CACCACCAGGTAATCGGTATGAGGAACTGTCAGGGAAGCTAAATGGCTATTCATCAGTAAGGGTGAATAAGCAATATAGG
TTAATTTTTAAGTGGGTTAACGGAAAGGCCGAGGACTTGTATCTCGACCCTCACAAATACTAA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 13049.98 Da
• Isoelectric Point: 6.4754

>AT112709 WP_000806445.1 NZ_CP032875:31842-32180 [Escherichia coli]
MKQATRKPTTVGDILLYEYLEPLELKINELAEILHVHRNTVSALVNNNRKLTMDMAYRLAKAFDTSVDFWINLQTAVDLW
EVENDMRVQEELSRINTAEKFISQRNLNKKAA

Nucleotide

• Length: 339 bp

>AT112709 NZ_CP032875:31842-32180 [Escherichia coli]
ATGAAACAGGCAACCAGAAAACCGACGACCGTAGGTGATATCCTTCTGTACGAATACCTGGAGCCGTTAGAGCTGAAGAT
CAACGAGTTAGCAGAAATACTTCATGTTCATCGTAACACCGTAAGTGCTCTTGTTAATAATAATCGTAAGCTAACGATGG
ATATGGCATATCGCCTGGCAAAAGCATTCGATACTTCTGTAGATTTTTGGATTAATCTTCAGACCGCAGTAGATCTGTGG
GAAGTCGAAAATGATATGCGCGTTCAGGAAGAGTTAAGTCGTATCAATACTGCTGAAAAATTTATTTCTCAGCGGAACCT
GAATAAAAAAGCAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A631VBQ3