Detailed information of TA system TA112708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 6153900..6154493
• Replicon: chromosome
• Accession: NZ_CP032871
• Organism: Pseudomonas syringae pv. actinidiae strain P220

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A0A2G7PD43
• Locus tag: D9N00_RS30425
• Protein ID: WP_003377943.1
• Coordinates: 6154215..6154493 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88B19
• Locus tag: D9N00_RS30420
• Protein ID: WP_005739893.1
• Coordinates: 6153900..6154205 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9N00_RS30380	6149121..6150305	+	1185	Protein_5440	IS4-like element ISPsy33 family transposase	-
D9N00_RS30385	6150356..6150688	-	333	WP_003377934.1	hypothetical protein	-
D9N00_RS30390	6150787..6151212	-	426	WP_003377936.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
D9N00_RS30400	6151455..6152792	-	1338	WP_003377937.1	ammonium transporter	-
D9N00_RS30405	6152827..6153165	-	339	WP_002555808.1	P-II family nitrogen regulator	-
D9N00_RS30415	6153585..6153866	+	282	WP_017682769.1	accessory factor UbiK family protein	-
D9N00_RS30420	6153900..6154205	-	306	WP_005739893.1	HigA family addiction module antidote protein	Antitoxin
D9N00_RS30425	6154215..6154493	-	279	WP_003377943.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D9N00_RS30430	6154664..6155578	-	915	WP_019332092.1	LysR family transcriptional regulator	-
D9N00_RS30435	6156063..6157583	+	1521	WP_024533279.1	amino acid ABC transporter permease/ATP-binding protein	-
D9N00_RS30440	6157608..6158480	+	873	WP_005613618.1	phytanoyl-CoA dioxygenase family protein	-
D9N00_RS30445	6158508..6159341	+	834	WP_005739898.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	6126370..6157583	31213

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10512.18 Da
• Isoelectric Point: 9.1490

>T112708 WP_003377943.1 NZ_CP032871:c6154493-6154215 [Pseudomonas syringae pv. actinidiae]
MIVSFKCVHTRYLFLQGKTRLWPSIKSVAERKLAMLDAATSILDLRSPPGNRLEVLDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T112708 NZ_CP032871:c6154493-6154215 [Pseudomonas syringae pv. actinidiae]
ATGATTGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTGGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGTGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGATGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11158.01 Da
• Isoelectric Point: 7.7509

>AT112708 WP_005739893.1 NZ_CP032871:c6154205-6153900 [Pseudomonas syringae pv. actinidiae]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Nucleotide

• Length: 306 bp

>AT112708 NZ_CP032871:c6154205-6153900 [Pseudomonas syringae pv. actinidiae]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGAGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A2G7PD43

Antitoxin

• AlphaFold DB: A0A0K8LYY6