Detailed information of TA system TA112651

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34104..34465
• Replicon: plasmid pINF237_03
• Accession: NZ_CP032836
• Organism: Klebsiella pneumoniae strain INF237

Toxin (Protein)

• Gene name: hok
• Uniprot ID: Q2VNZ0
• Locus tag: D9K64_RS27935
• Protein ID: WP_000775238.1
• Coordinates: 34304..34465 (+)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	34104..34238 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D9K64_RS27895	29243..29473	+	231	WP_115233630.1	hypothetical protein	-
D9K64_RS27900	29525..30346	+	822	WP_073971918.1	DUF3560 domain-containing protein	-
D9K64_RS27905	30313..30381	+	69	Protein_40	DUF905 family protein	-
D9K64_RS27910	30447..32405	+	1959	WP_044243914.1	ParB/RepB/Spo0J family partition protein	-
D9K64_RS27915	32460..32894	+	435	WP_032307973.1	conjugation system SOS inhibitor PsiB	-
D9K64_RS27920	32891..33610	+	720	WP_115233631.1	plasmid SOS inhibition protein A	-
D9K64_RS27925	33610..34128	+	519	WP_001178556.1	hypothetical protein	-
-	34104..34238	+	135	NuclAT_0	-	Antitoxin
-	34104..34238	+	135	NuclAT_0	-	Antitoxin
-	34104..34238	+	135	NuclAT_0	-	Antitoxin
-	34104..34238	+	135	NuclAT_0	-	Antitoxin
D9K64_RS27935	34304..34465	+	162	WP_000775238.1	type I toxin-antitoxin system Hok family toxin	Toxin
D9K64_RS29680	34769..35018	-	250	Protein_46	hypothetical protein	-
D9K64_RS27955	35319..35606	+	288	WP_000107526.1	hypothetical protein	-
D9K64_RS27960	35725..36546	+	822	WP_142675327.1	DUF945 domain-containing protein	-
D9K64_RS27970	36841..37443	-	603	WP_115233634.1	transglycosylase SLT domain-containing protein	-
D9K64_RS27975	37774..38157	+	384	WP_001151564.1	relaxosome protein TraM	-
D9K64_RS27980	38350..39036	+	687	WP_129112405.1	PAS domain-containing protein	-
D9K64_RS27985	39130..39357	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..76772	76772

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5978.22 Da
• Isoelectric Point: 8.5101

>T112651 WP_000775238.1 NZ_CP032836:34304-34465 [Klebsiella pneumoniae]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 162 bp

>T112651 NZ_CP032836:34304-34465 [Klebsiella pneumoniae]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTGATATTCACACTCCTGACCCG
GAATCGCCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAAGTTACGGCAACAATGGCTTACGAATCCGGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 135 bp

>AT112651 NZ_CP032836:34104-34238 [Klebsiella pneumoniae]
CAGGGTGCAGACATATGGGCTATGATGTGCCCGGCGCTTGAGGCTTTTTGCCTCATGACGTGAAGGTGGTTTGTTACCGT
GTTGTGTGGCAGAAAGCAGAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: A0A7Z7YI12

Antitoxin