Detailed information of TA system TA112585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1646244..1646761
• Replicon: chromosome
• Accession: NZ_CP032821
• Organism: Staphylococcus aureus strain JDFM SA01

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: D5S13_RS08000
• Protein ID: WP_000113262.1
• Coordinates: 1646495..1646761 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: D5S13_RS07995
• Protein ID: WP_000584499.1
• Coordinates: 1646244..1646495 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5S13_RS07965 (D5S13_01527)	1641942..1642661	+	720	WP_000479576.1	amino acid ABC transporter permease	-
D5S13_RS07970 (D5S13_01528)	1642658..1643389	+	732	WP_000615470.1	amino acid ABC transporter ATP-binding protein	-
D5S13_RS07975 (D5S13_01529)	1643486..1644736	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
D5S13_RS07980 (D5S13_01530)	1644991..1645353	+	363	WP_263258424.1	DUF4467 domain-containing protein	-
D5S13_RS07985 (D5S13_01531)	1645372..1645971	+	600	WP_000162813.1	DsbA family protein	-
D5S13_RS07990	1645978..1646079	+	102	WP_001791744.1	hypothetical protein	-
D5S13_RS07995 (D5S13_01532)	1646244..1646495	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D5S13_RS08000 (D5S13_01533)	1646495..1646761	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
D5S13_RS08005	1646796..1646867	+	72	Protein_1573	hypothetical protein	-
D5S13_RS08010 (D5S13_01534)	1647056..1648606	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
D5S13_RS08015 (D5S13_01535)	1648792..1649259	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
D5S13_RS08020 (D5S13_01536)	1649343..1649525	+	183	WP_000230294.1	hypothetical protein	-
D5S13_RS08025 (D5S13_01537)	1649742..1650566	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
D5S13_RS08030 (D5S13_01538)	1650799..1651335	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T112585 WP_000113262.1 NZ_CP032821:1646495-1646761 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T112585 NZ_CP032821:1646495-1646761 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT112585 WP_000584499.1 NZ_CP032821:1646244-1646495 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT112585 NZ_CP032821:1646244-1646495 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60