Detailed information of TA system TA112582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 1589970..1590493
• Replicon: chromosome
• Accession: NZ_CP032821
• Organism: Staphylococcus aureus strain JDFM SA01

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: D5S13_RS07735
• Protein ID: WP_000074046.1
• Coordinates: 1590227..1590493 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A2K4AGF2
• Locus tag: D5S13_RS07730
• Protein ID: WP_000587615.1
• Coordinates: 1589970..1590227 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5S13_RS07710 (D5S13_01479)	1585065..1586258	+	1194	WP_000675403.1	MFS transporter	-
D5S13_RS07715 (D5S13_01480)	1586482..1587231	-	750	WP_000019956.1	CPBP family lipoprotein N-acylation protein LnsB	-
D5S13_RS07720 (D5S13_01481)	1587421..1588998	+	1578	WP_000143490.1	FMN-binding glutamate synthase family protein	-
D5S13_RS07725 (D5S13_01482)	1589148..1589612	-	465	WP_000736689.1	DUF1307 domain-containing protein	-
D5S13_RS07730 (D5S13_01483)	1589970..1590227	+	258	WP_000587615.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
D5S13_RS07735 (D5S13_01484)	1590227..1590493	+	267	WP_000074046.1	Txe/YoeB family addiction module toxin	Toxin
D5S13_RS07740 (D5S13_01485)	1590806..1591882	+	1077	WP_001076667.1	M42 family metallopeptidase	-
D5S13_RS07745 (D5S13_01486)	1592130..1592792	+	663	WP_000923528.1	ATP-binding cassette domain-containing protein	-
D5S13_RS07750 (D5S13_01487)	1592785..1593558	+	774	WP_000072148.1	iron export ABC transporter permease subunit FetB	-
D5S13_RS07755 (D5S13_01488)	1593910..1595094	+	1185	WP_000115415.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-67)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10658.27 Da
• Isoelectric Point: 10.1384

>T112582 WP_000074046.1 NZ_CP032821:1590227-1590493 [Staphylococcus aureus]
MSNYTVKIKNSAKSDLKKIKHSYLKKPFLEIVETLKNDPYKITQSFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T112582 NZ_CP032821:1590227-1590493 [Staphylococcus aureus]
ATGAGCAATTATACGGTTAAGATTAAAAATTCGGCGAAATCAGATTTAAAGAAAATAAAACATTCTTATTTAAAAAAGCC
ATTTTTAGAAATTGTTGAGACTTTAAAAAATGATCCGTATAAAATAACACAATCCTTTGAAAAATTAGAGCCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACTGTAGATGATCGAAATAAAGAAGTATTAATA
TTATCGGCGTGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9746.68 Da
• Isoelectric Point: 4.1111

>AT112582 WP_000587615.1 NZ_CP032821:1589970-1590227 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLDDWKSIQETIYLESTGTMDKVRDREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT112582 NZ_CP032821:1589970-1590227 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGATGATTGGAAAAGTATACAAGAAACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGATAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2K4AGF2