Detailed information of TA system TA112561

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2297338..2297709
• Replicon: chromosome
• Accession: NZ_CP032811
• Organism: Escherichia coli strain ERL03-1416

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A891STD1
• Locus tag: D8Z67_RS11765
• Protein ID: WP_042853000.1
• Coordinates: 2297515..2297709 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2297338..2297516 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z67_RS11725	2292780..2293436	-	657	WP_000011656.1	carbon-nitrogen hydrolase family protein	-
D8Z67_RS11730	2293538..2293768	-	231	WP_000916763.1	DNA polymerase III subunit theta	-
D8Z67_RS11735	2293907..2294281	+	375	WP_000168751.1	CopC domain-containing protein YobA	-
D8Z67_RS11740	2294285..2295157	+	873	WP_000879314.1	copper homeostasis membrane protein CopD	-
D8Z67_RS11745	2295170..2295511	+	342	WP_000976483.1	YebY family protein	-
D8Z67_RS11750	2295904..2296980	-	1077	WP_045893699.1	phage integrase Arm DNA-binding domain-containing protein	-
D8Z67_RS11755	2296946..2297227	-	282	WP_001311878.1	excisionase	-
D8Z67_RS11760	2297334..2297522	-	189	WP_001356607.1	DUF1187 family protein	-
-	2297338..2297516	+	179	NuclAT_0	-	Antitoxin
-	2297338..2297516	+	179	NuclAT_0	-	Antitoxin
-	2297338..2297516	+	179	NuclAT_0	-	Antitoxin
-	2297338..2297516	+	179	NuclAT_0	-	Antitoxin
D8Z67_RS11765	2297515..2297709	-	195	WP_042853000.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
D8Z67_RS11770	2297766..2298575	-	810	WP_000166313.1	recombination protein RecT	-
D8Z67_RS11775	2298568..2301237	-	2670	WP_000102194.1	exodeoxyribonuclease VIII	-
D8Z67_RS11780	2301318..2301494	-	177	WP_000245534.1	hypothetical protein	-
D8Z67_RS11785	2301488..2301709	-	222	WP_000560228.1	killing protein KilR	-
D8Z67_RS11790	2301756..2302607	-	852	WP_062882198.1	Rha family phage regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2275459..2357017	81558

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7019.87 Da
• Isoelectric Point: 8.6419

>T112561 WP_042853000.1 NZ_CP032811:c2297709-2297515 [Escherichia coli]
MRYDDVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T112561 NZ_CP032811:c2297709-2297515 [Escherichia coli]
ATGAGATATGACGATGTTAAACCATGTCCGTTTTGTGGTTGTCCATCTGTTACAGTGAAAGCAATTTCAGGATATTACCG
GGCAAAATGCAACGGATGCGAATCCCGAACTGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CTACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT112561 NZ_CP032811:2297338-2297516 [Escherichia coli]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAATTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCTTTTT
CAATAGTGGCAGTTATTTT

Structures

Antitoxin