Detailed information of TA system TA112540

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 22250..22520
• Replicon: plasmid pERL04-3476-2
• Accession: NZ_CP032810
• Organism: Escherichia coli strain ERL04-3476

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: D8Z68_RS29120
• Protein ID: WP_001312861.1
• Coordinates: 22362..22520 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	22250..22313 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D8Z68_RS29075	17478..17702	-	225	WP_001427866.1	hypothetical protein	-
D8Z68_RS29095	18044..18571	+	528	WP_000290793.1	single-stranded DNA-binding protein	-
D8Z68_RS29100	18627..18860	+	234	WP_000006004.1	DUF905 domain-containing protein	-
D8Z68_RS29105	18919..20877	+	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
D8Z68_RS29110	20932..21366	+	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
D8Z68_RS29115	21363..22082	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
D8Z68_RS29585	22094..22282	-	189	WP_001299721.1	hypothetical protein	-
-	22094..22318	+	225	NuclAT_0	-	-
-	22094..22318	+	225	NuclAT_0	-	-
-	22094..22318	+	225	NuclAT_0	-	-
-	22094..22318	+	225	NuclAT_0	-	-
-	22250..22313	-	64	-	-	Antitoxin
D8Z68_RS29120	22362..22520	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
D8Z68_RS29590	22874..23098	-	225	WP_001340580.1	hypothetical protein	-
D8Z68_RS29145	23439..23726	+	288	WP_000107537.1	hypothetical protein	-
D8Z68_RS29150	23847..24668	+	822	WP_001234469.1	DUF945 domain-containing protein	-
D8Z68_RS29155	24965..25612	-	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
D8Z68_RS29160	25889..26272	+	384	WP_000124983.1	relaxosome protein TraM	-
D8Z68_RS29165	26463..27149	+	687	WP_000332484.1	PAS domain-containing protein	-
D8Z68_RS29170	27243..27470	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..61441	61441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T112540 WP_001312861.1 NZ_CP032810:22362-22520 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T112540 NZ_CP032810:22362-22520 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT112540 NZ_CP032810:c22313-22250 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin